Abstract: The importance of cytotoxic T-lymphocyte（CTL）against malaria parasite in pre-erythrocytic stage has been presented in relevant researches. In order to investigate whether one CTL epitope（YLNKIQNSL）involved in a chimeric pre-erythrocytic stage vaccine candidate of Plasmodium falciparum which was expressed and purified in the laboratory can stimulate in vivo CTL response，HLA-A*0201 transgenic mice were immunized with this vaccine candidate. Enzyme-linked immunosorbent spot（ELISPOT）assay was performed on the splenocytes from the immunized transgenic mice. Positive result indicated that this CTL epitope can be in vivo processed and correctly presented.

Key words: Plasmodium falciparum, Vaccine, Pre-erythrocytic stage, CTL, ELISPOT assay