Interaction between mouse macrophages and protoscolex of Echinococcus granulosus in vitro

doi:10.12140/j.issn.1000-7423.2025.01.009

Abstract

Abstract:

Objective To explore the cytotoxic effect of mouse immune cells on the Echinococcus granulosus protoscolex and understand the types of immune cells involved and the cytokines secretion changes. Methods Healthy C57BL/6 mice were used to extract splenocytes and peritoneal macrophages. The protoscoleces from sheep E. granulosus cysts were collected and grouped (3 000 per group). To select the immune cell type exhibiting a stronger inhibitory effect on the protoscoleces, macrophage co-culture group and splenocyte co-culture group were co-cultured with 6 × 10⁶ macrophages or splenocytes, respectively. To choose an optimal cell count, co-culture group 1-5 were co-cultured with 1.2 × 10⁶, 2.4 × 10⁶, 4.8 × 10⁶, 7.2 × 10⁶ and 9.6 × 10⁶ immune cells, respectively. The co-culture system was established. The E. granulosus cyst fluid and tumor necrosis factor-α (TNF-α) inhibitor were added into the co-culture system respectively, and the activity of protoscoleces and the changes in concentration of TNF-α, interleukin 6 (IL-6), IL-10 and transforming growth factor-β (TGF-β) in the co-culture supernatant were observed. Eosin staining was used to detect the activity of protoscoleces, the dichlorodihydrofluorescein diacetate (DCFH-DA) method was used to measure the of reactive oxygen species levels, the JC-1 method was used to assess mitochondrial membrane potential, Western blotting was performed to detect the expression levels of the Bcl-2 associated X protein (Bax) and cysteinyl aspartate specific proteinase 3 (caspase-3), and ELISA was used to measure the concentration of cytokines in the culture supernatant. Independent samples t-test was used for comparisons between two groups and one-way ANOVA was used for multiple groups. Results On day 6 of co-culture, the protoscoleces activity in the macrophage co-culture group and the splenocyte co-culture group was (25.07 ± 0.40)% and (76.18 ± 0.31)%, respectively. The protoscoleces activity in the macrophage co-culture group was lower than that in the splenocyte co-culture group at all time points (F = 564.20, P < 0.05). On day 4 of co-culture, the relative fluorescent intensity of reactive oxygen species in the protoscoleces of the macrophage co-culture group was 32.20 ± 7.85, which was higher than that of the splenocyte co-culture group (12.44 ± 2.93) (t = 7.07, P < 0.05). On day 6 of co-culture, the relative expression levels of caspase-3 protein in the macrophage co-culture group and the splenocyte co-culture group were 1.28 ± 0.02 and 1.16 ± 0.02, respectively, and the relative expression levels of Bax protein were 1.29 ± 0.01 and 0.46 ± 0.01, respectively. The relative expression levels of caspase-3 and Bax protein in the macrophage co-culture group were higher than those in the splenocyte co-culture group at all time points (F = 55.87, 167.20; both P < 0.05). Macrophages exhibited a stronger inhibitory effect on protoscoleces than splenocytes. On day 6 of co-culture, the relative fluorescent intensities of mitochondrial membrane potential in protoscoleces from co-culture groups 1-5 were 20.15 ± 8.96, 24.40 ± 9.71, 48.41 ± 10.20, 94.62 ± 8.72 and 112.85 ± 24.23, respectively, all of which were higher than that of the protoscolex control group (2.50 ± 1.02) (F = 26.18, P < 0.01). On day 6 of co-culture, the relative expression levels of caspase-3 protein in protoscoleces from co-culture group 4 and 5 were 1.35 ± 0.03 and 1.49 ± 0.05, respectively, which were higher than that of the protoscolex control group (0.28 ± 0.01) (t = 17.03, 10.60; both P < 0.05). The relative expression levels of Bax protein in protoscoleces from co-culture groups 4 and 5 were 1.34 ± 0.01 and 1.38 ± 0.04, respectively, which were higher than that of the protoscolex control group (0.78 ± 0.04) (t = 6.68, 6.46; both P < 0.05). On day 4 of co-culture, the concentrations of TNF-α, IL-6 and TGF-β in the supernatants of co-culture group 4 were (240.90 ± 17.29), (435.90 ± 12.33) and (137.10 ± 6.62) pg/ml, respectively, all of which were higher than those in the cell control group [(42.02 ± 0.52), (65.72 ± 1.91), (24.72 ± 1.78) pg/ml] (t = 54.52, 15.97, 17.59; all P < 0.05). The concentration of IL-10 in the supernatant of co-culture group 4 was (42.16 ± 1.45) pg/ml, which was not significantly different from that of the cell control group [(45.64 ± 1.03) pg/ml] (t = 1.29, P > 0.05). The concentrations of TNF-α, IL-6, IL-10 and TGF-β in the supernatants of the co-culture groups at all time points were higher than those in the cell control group (F = 294.66, 450.50, 687.72, 660.15; all P < 0.05). On days 1, 3, 5 and 7 of co-culture, the relative fluorescent intensities of mitochondrial membrane potential in protoscoleces from the cyst fluid group were 4.46 ± 1.25, 4.33 ± 0.39, 4.89 ± 0.77 and 7.97 ± 0.62, respectively, all of which were lower than those in the macrophage group (5.67 ± 1.72, 13.60 ± 0.50, 35.28 ± 5.65, 77.50 ± 9.60) (F = 115.90, P < 0.01). On day 6 of co-culture, the concentrations of TNF-α, IL-6, IL-10 and TGF-β in the supernatant of the cyst fluid group were (64.12 ± 2.65), (1 049.65 ± 25.70), (230.30 ± 12.98) and (138.57 ± 13.71) pg/ml, respectively, and those in the macrophage group were (41.61 ± 1.31), (68.00 ± 0.42), (56.15 ± 6.43) and (32.94 ± 4.90) pg/ml, respectively. The concentrations of TNF-α, IL-6, IL-10 and TGF-β in the supernatants of the cyst fluid group at all time points were higher than those in the macrophage group (F = 289.80, 366.50, 145.40, 32.94; all P < 0.05). On day 7 of co-culture, the protoscoleces activity in the macrophage group and the inhibitor group was (21.18 ± 1.61)% and (94.31 ± 2.58)%, respectively. The protoscoleces activity in the inhibitor group was higher than that in the macrophage group at all time points (F = 1 810.00, P < 0.05). On days 2, 4 and 6 of co-culture, the concentrations of TNF-α in the inhibitor group were (33.55 ± 7.48), (13.78 ± 4.96) and (19.20 ± 0.69) pg/ml, respectively, all of which were lower than those in the macrophage group [(209.24 ± 9.90), (209.47 ± 10.55), (211.36 ± 13.66) pg/ml] (t = 33.16, 30.46, 23.76; all P < 0.05). Conclusion Macrophages co-cultured in vitro with the E. granulosus protoscoleces could express cytokines such as TNF-α, which inhibits the activity of the protoscoleces and promotes their apoptosis. Cyst fluid from E. granulosus and TNF-α inhibitors could reduce the secretion of TNF-α by macrophages, thereby alleviating the killing effect of macrophages on the protoscoleces.

Key words: Echinococcus granulosus, Macrophages, TNF-α, Caspase-3, Co-culture

CLC Number:

R383.33

LI Guang, JIANG Huijiao, DU Yunfeng, SHU Min, LUO Yumeng, ZHU Lingyi, CHEN Xueling, WU Xiangwei. Interaction between mouse macrophages and protoscolex of Echinococcus granulosus in vitro[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 52-60.

Figures/Tables 8

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Table 2

The cytokines in culture supernatant of protoscoleces co-culture with different numbers of macrophages in vitro

组别 Group	细胞因子浓度/pg·ml^-1 Cytokines concentration/pg·ml^-1
组别 Group	TNF-α	IL-6	IL-10	TGF-β
共培养第2天 Day 2 of co-culture
原头节对照组 Protoscolece control group	14.88 ± 0.34	7.25 ± 0.07	16.50 ± 0.91	31.63 ± 2.24
细胞对照组 Cell control group	39.07 ± 2.01	66.72 ± 4.62	43.90 ± 5.57	34.23 ± 3.38
共培养1组 Co-culture group 1	67.58 ± 2.99	412.85 ± 19.30	123.68 ± 9.17	97.27 ± 16.05
共培养2组 Co-culture group 2	71.34 ± 5.52	420.29 ± 18.47	103.22 ± 5.47	139.29 ± 9.18
共培养3组 Co-culture group 3	85.16 ± 5.02	429.51 ± 23.67	96.97 ± 6.69	288.82 ± 2.68
共培养4组 Co-culture group 4	161.06 ± 15.61	446.57 ± 28.56	64.73 ± 5.38	320.53 ± 14.26
共培养5组 Co-culture group 5	169.30 ± 14.24	487.11 ± 13.55	49.48 ± 6.66	338.39 ± 11.57
共培养第4天 Day 4 of co-culture
原头节对照组 Protoscolece control group	14.61 ± 0.69	7.21 ± 0.11	16.63 ± 0.74	32.31 ± 3.32
细胞对照组 Cell control group	41.92 ± 0.88	65.67 ± 3.81	57.44 ± 4.95	34.89 ± 5.62
共培养1组 Co-culture group 1	300.12 ± 12.04	363.69 ± 19.74	107.45 ± 5.94	54.07 ± 5.35
共培养2组 Co-culture group 2	317.91 ± 20.42	381.70 ± 23.13	128.02 ± 8.55	64.93 ± 6.61
共培养3组 Co-culture group 3	327.48 ± 40.88	380.24 ± 19.46	87.13 ± 4.92	154.65 ± 11.95
共培养4组 Co-culture group 4	262.83 ± 17.10	406.98 ± 24.44	37.42 ± 3.59	187.83 ± 12.43
共培养5组 Co-culture group 5	215.82 ± 11.48	420.11 ± 25.13	44.75 ± 8.71	242.70 ± 32.05
共培养第6天 Day 6 of co-culture
原头节对照组 Protoscolece control group	14.51 ± 0.69	7.22 ± 0.12	23.71 ± 1.20	26.14 ± 2.52
细胞对照组 Cell control group	42.02 ± 0.52	65.72 ± 1.91	45.64 ± 1.03	24.72 ± 1.78
共培养1组 Co-culture group 1	163.17 ± 4.64	378.35 ± 19.91	161.42 ± 6.45	45.47 ± 12.70
共培养2组 Co-culture group 2	178.04 ± 23.32	391.70 ± 25.34	262.55 ± 12.87	46.38 ± 7.57
共培养3组 Co-culture group 3	315.21 ± 24.50	405.24 ± 14.10	106.24 ± 4.94	74.56 ± 5.52
共培养4组 Co-culture group 4	240.90 ± 17.29	435.90 ± 12.33	42.16 ± 1.45	137.10 ± 6.62
共培养5组 Co-culture group 5	199.27 ± 8.41	432.44 ± 24.30	42.74 ± 3.23	184.60 ± 11.01

Table 2

Fig. 5

Table 3

References 27

[1]	Wang LY, Qin M, Gavotte L, et al. Societal drivers of human echinococcosis in China[J]. Parasit Vectors, 2022, 15(1): 385.
[2]	Siracusano A, Teggi A, Ortona E. Human cystic echinococcosis: Old problems and new perspectives[J]. Interdiscip Perspect Infect Dis, 2009, 2009: 474368.
[3]	Wen H, Vuitton L, Tuxun T, et al. Echinococcosis: Advances in the 21st century[J]. Clin Microbiol Rev, 2019, 32(2): e00075-18.
[4]	Peters L, Burkert S, Grüner B. Parasites of the liver―epidemiology, diagnosis and clinical management in the European context[J]. J Hepatol, 2021, 75(1): 202-218.
[5]	Gottstein B, Soboslay P, Ortona E, et al. Immunology of alveolar and cystic echinococcosis (AE and CE)[J]. Adv Parasitol, 2017, 96: 1-54.
[6]	Wang XP, Lin RL, Fu CX, et al. Echinococcus granulosus cyst fluid inhibits inflammatory responses through inducing histone demethylase KDM5B in macrophages[J]. Parasit Vectors, 2023, 16(1): 321.
[7]	Daniel-Mwambete K, Torrado S, Cuesta-Bandera C, et al. The effect of solubilization on the oral bioavailability of three benzimidazole carbamate drugs[J]. Int J Pharm, 2004, 272(1/2): 29-36.
[8]	Díaz á. Immunology of cystic echinococcosis (hydatid disease)[J]. Br Med Bull, 2017, 124(1): 121-133.
[9]	De Stefano L, Pallavicini FB, Mauric E, et al. Tumor necrosis factor-α inhibitor-related autoimmune disorders[J]. Autoimmun Rev, 2023, 22(7): 103332.
[10]	Singh N, Kumar R, Engwerda C, et al. Tumor necrosis factor alpha neutralization has no direct effect on parasite burden, but causes impaired IFN-γ production by spleen cells from human visceral leishmaniasis patients[J]. Cytokine, 2016, 85: 184-190.
[11]	Saxton RA, Tsutsumi N, Su LL, et al. Structure-based decoupling of the pro- and anti-inflammatory functions of interleukin-10[J]. Science, 2021, 371(6535): eabc8433.
[12]	Yasen A, Ran B, Wang ML, et al. Roles of immune cells in the concurrence of Echinococcus granulosus sensu lato infection and hepatocellular carcinoma[J]. Exp Parasitol, 2022, 240: 108321.
[13]	Canton M, Sánchez-Rodríguez R, Spera I, et al. Reactive oxygen species in macrophages: Sources and targets[J]. Front Immunol, 2021, 12: 734229.
[14]	谭小武, 姜慧娇, 俞晓凡, 等. 黄腐酚对体外泡状棘球蚴原头节活性的影响[J]. 中国病原生物学杂志, 2021, 16(2): 131-136.
	Tan XW, Jiang HJ, Yu XF, et al. Effect of xanthohumol on the activity of protoscoleces of Echinococcus multilocularis in vitro[J]. J Pathog Biol, 2021, 16(2): 131-136. (in Chinese)
[15]	Casulli A, Siles-Lucas M, Tamarozzi F. Echinococcus granulosus sensu lato[J]. Trends Parasitol, 2019, 35(8): 663-664.
[16]	温浩, 吐尔干艾力·阿吉, 邵英梅, 等. 棘球蚴病防治成就及面临的挑战[J]. 中国寄生虫学与寄生虫病杂志, 2015, 33(6): 466-471.
	Wen H, Tuerganaili A, Shao YM, et al. Research achievements and challenges for echinococcosis control[J]. Chin J Parasitol Parasit Dis, 2015, 33(6): 466-471. (in Chinese)
[17]	Leonard WJ, Lin JX. Strategies to therapeutically modulate cytokine action[J]. Nat Rev Drug Discov, 2023, 22(10): 827-854.
[18]	Beyhan YE, Albayrak C, Guven I. IL-4, IL-10, TNF-α and IFN-γ cytokine levels in patients with cystic echinococcosis and fascioliasis[J]. Iran J Public Health, 2022, 51(6): 1339-1347.
[19]	Manohar SM. At the crossroads of TNF-α signaling and cancer[J]. Curr Mol Pharmacol, 2024, 17(1): e060923220758.
[20]	Mondragón-de-la-Pe?a C, Ramos-Solís S, Barbosa-Cisneros O, et al. Echinococcus granulosus down regulates the hepatic expression of inflammatory cytokines IL-6 and TNF alpha in BALB/c mice[J]. Parasite, 2002, 9(4): 351-356.
[21]	Uoselis L, Nguyen TN, Lazarou M. Mitochondrial degradation: Mitophagy and beyond[J]. Mol Cell, 2023, 83(19): 3404-3420.
[22]	Eddie Ip WK, Hoshi N, Shouval DS, et al. Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages[J]. Science, 2017, 356(6337): 513-519.
[23]	Wu JC, Zhu YQ, Zhou LM, et al. Parasite-derived excre-tory-secretory products alleviate gut microbiota dysbiosis and improve cognitive impairment induced by a high-fat diet[J]. Front Immunol, 2021, 12: 710513.
[24]	Ouaissi A. Regulatory cells and immunosuppressive cytokines: Parasite-derived factors induce immune polarization[J]. J Biomed Biotechnol, 2007, 2007(4): 94971.
[25]	逯君霞, 许军英, 赵彬, 等. 细粒棘球蚴感染诱导巨噬细胞表达CD73和A2AR抑制炎症反应[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 559-566, 572.
	Lu JX, Xu JY, Zhao B, et al. Echinococcus granulosus infection induces macrophages to express CD73 and A2AR to suppress inflammatory response[J]. Chin J Parasitol Parasit Dis, 2023, 41(5): 559-566, 572. (in Chinese)
[26]	Hwang MA, Won M, Im JY, et al. TNF-α secreted from macrophages increases the expression of prometastatic integrin αV in gastric cancer[J]. Int J Mol Sci, 2022, 24(1): 376.
[27]	Skytthe MK, Graversen JH, Moestrup SK. Targeting of CD163⁺ macrophages in inflammatory and malignant diseases[J]. Int J Mol Sci, 2020, 21(15): 5497.

共培养时间/d Time of co-culture/d	原头节活性/% Protoscolex activity/%			t₁值^a t₁ value^a	P₁值^a P₁ value^a	t₂值^b t₂ value^b	P₂值^b P₂ value^b
共培养时间/d Time of co-culture/d	原头节对照组 Protoscolece control group	巨噬细胞共培养组 Macrophage co-culture group	脾细胞共培养组 Splenocyte co-culture group	t₁值^a t₁ value^a	P₁值^a P₁ value^a	t₂值^b t₂ value^b	P₂值^b P₂ value^b
0	100 ± 0	97.53 ± 1.04	97.95 ± 1.03	4.12	< 0.05	3.45	< 0.05
2	100 ± 0	84.00 ± 1.26	91.65 ± 1.33	22.07	< 0.05	10.86	< 0.05
4	100 ± 0	32.48 ± 2.23	82.66 ± 2.18	52.43	< 0.05	13.78	< 0.05
6	98.03 ± 1.26	25.07 ± 0.40	76.18 ± 0.31	95.59	< 0.05	29.29	< 0.05
7	96.36 ± 0.90	20.30 ± 1.33	57.54 ± 1.47	32.54	< 0.05	39.08	< 0.05

共培养时间/d Time of co-culture/d	原头节活性/% Protoscolex activity/%			t₁值^a t₁ value^a	P₁值^a P₁ value^a	t₂值^b t₂ value^b	P₂值^b P₂ value^b
共培养时间/d Time of co-culture/d	原头节对照组 Protoscolece control group	巨噬细胞共培养组 Macrophage co-culture group	脾细胞共培养组 Splenocyte co-culture group	t₁值^a t₁ value^a	P₁值^a P₁ value^a	t₂值^b t₂ value^b	P₂值^b P₂ value^b
0	100 ± 0	97.53 ± 1.04	97.95 ± 1.03	4.12	< 0.05	3.45	< 0.05
2	100 ± 0	84.00 ± 1.26	91.65 ± 1.33	22.07	< 0.05	10.86	< 0.05
4	100 ± 0	32.48 ± 2.23	82.66 ± 2.18	52.43	< 0.05	13.78	< 0.05
6	98.03 ± 1.26	25.07 ± 0.40	76.18 ± 0.31	95.59	< 0.05	29.29	< 0.05
7	96.36 ± 0.90	20.30 ± 1.33	57.54 ± 1.47	32.54	< 0.05	39.08	< 0.05