›› 2010, Vol. 28 ›› Issue (2): 10-132.

• 论著 • Previous Articles     Next Articles

Soluble Proteins of the Plerocercoid of Spirometra mansoni

JIANG Hong-tao1，2，CHEN Yan1 *，TANG Gui-wen1，CHEN Lu1   

  1. 1 Department of Parasitology, Guiyang Medical College，Guiyang 550004，China；2 Guizhou Publishing Group，Guiyang 550004，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-30 Published:2010-04-30
  • Contact: CHEN Yan

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Abstract: Objective To analyze soluble proteins of the plerocercoid of Spirometra mansoni. Methods The total protein of the plerocercoid of S. mansoni was separated by SDS-PAGE and two-dimensional electrophoresis （2-DE）. Western blotting was performed to find out distinct antigens by sera of plerocercoid-infected mice. Results A total of 33 protein bands were separated by SDS-PAGE （Mr 13 800-145 400）. Four of them were high-abundance proteins with Mr 26 500, Mr 37 600, Mr 88 200, and Mr 130 200, respectively. At least two protein bands of Mr 31 600 and 37 600 reacted with the infected mice sera. 367 protein spots were detected on 2-DE gel, among which about 67% proteins were found within Mr 18 840-46 800 and isoelectric point （pI） 4-7. Western blotting showed 30 antigen spots specifically reacting with sera from mice infected by S. mansoni. Conclusion Two protein bands and thirty protein spots are specific acidic proteins of S. mansoni plerocercoid.

Key words: Spirometra mansoni, Plerocercoid, Antigen, SDS-PAGE, Western blotting, Two-dimensional electrophoresis