Preparation and Identification of Monoclonal Antibodies against the Region II+ Motif in Circumsporozoite Protein of Plasmodium falciparum

›› 2004, Vol. 22 ›› Issue (2): 5-85.

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Preparation and Identification of Monoclonal Antibodies against the Region II⁺ Motif in Circumsporozoite Protein of Plasmodium falciparum

ZHANG Rui-juan,ZHU Huai-min,LI Xiang-yu

Department of Etiological Biology,Second Military Medical University,Shanghai 200433,China

Received:1900-01-01 Revised:1900-01-01 Online:2004-04-30 Published:2004-04-30
Contact: ZHU Huai-min

Abstract

Abstract: Objective To develop and identify the monoclonal antibodies (McAbs) against Region II⁺ motif in circumsporozoite protein of Plasmodium falciparum. Methods BALB/c mice were immunized with 12 peptides within Region Ⅱ⁺ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained. Results ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II⁺.IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii. Conclusion McAbs obtained can probe the Region II⁺ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species.

Key words: Plasmodium, monoclonal antibody, circumsporozoite protein, Region II⁺

ZHANGRui-juan;ZHUHuai-min;LIXiang-yu. Preparation and Identification of Monoclonal Antibodies against the Region II⁺ Motif in Circumsporozoite Protein of Plasmodium falciparum[J]. , 2004, 22(2): 5-85.

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Preparation and Identification of Monoclonal Antibodies against the Region II⁺ Motif in Circumsporozoite Protein of Plasmodium falciparum

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