Interpretation of the Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods

doi:10.12140/j.issn.1000-7423.2024.04.014

Abstract

Abstract:

The Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods (T/SPMA 004-2023) (referred to as the Criteria) was compiled following the Social Organization Standardization-Part 1: Guidelines for Good Practice (GB/T 20004.1-2016) and Standardization Working Guidelines (GB/T 1.1-2009). The Criteria is composed of seven chapters, including “the range of application” “normative reference documents” “terms and definitions” “instruments and equipment” “reagent and materials” “detection steps” and “waste disposal and pollution prevention measures”. One informative appendix (technical principle) and four normative appendices (two detection methods, reagent preparation and sample preparation). The Criteria was issued by Shanghai Preventive Medicine Association through No.34 of SJPM in 2023. The Criteria provides for the technical reference for normative operations and the quality of sample testing in disease control institutions and medical institutions. And the Criteria makes up for the deficiency of specific operating steps in nucleic acid detection of Criteria for Diagnosis of Malaria (WS 259-2015).

Key words: Plasmodium, Nucleic acid, PCR, Criteria

CLC Number:

R382.31

WANG Zhenyu, JIANG Li, YU Qing, ZHANG Yaoguang, WU Huanyu, CHEN Jian, ZHU Min. Interpretation of the Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 521-524.

References 11

[1]	World Health Organization. World malaria report 2023[R]. Geneva: WHO, 2023: 19.
[2]	WHO malaria-free certification. From 30 million cases to zero: China is certified malaria-free by WHO[EB/OL]. (2021-06-30)[2023-02-02]. https://www.who.int/news/item/30-06-2021-from-30-million-cases-to-zero-china-is-certified-malaria-free-by-who.
[3]	Zhu M, Cai L, Wu HY, et al. Mid-term assessment report of Malaria Elimination Action Plan in Shanghai[J]. China Trop Med, 2018, 18(4): 297-302. (in Chinese)
	(朱民, 蔡黎, 吴寰宇, 等. 上海市消除疟疾行动计划中期评估报告[J]. 中国热带医学, 2018, 18(4): 297-302.) doi: 10.13604/j.cnki.46-1064/r.2018.04.01
[4]	Jiang L, Wang ZY, Zhang YG, et al. Analysis on the application of three methods for malaria diagnosis[J]. Chin J Parasitol Parasit Dis, 2017, 35(1): 53-58. (in Chinese)
	(江莉, 王真瑜, 张耀光, 等. 3种疟疾检测方法的应用分析[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(1): 53-58.)
[5]	National Health and Planning Commission of the People’s Republic of China. Diagnosis of malaria: WS 259-2015[S]. Beijing: Standards Press of China, 2016: 1-12. (in Chinese)
	(中华人民共和国国家卫生和计划生育委员会. 疟疾的诊断: WS 259—2015[S]. 北京: 中国标准出版社, 2016: 1-12.)
[6]	Jiang L, Zhang YG, Cai L, et al. Establishment and application of multiplex PCR for co-detection of genus- and species-specific malaria parasites[J]. Chin J Parasitol Parasit Dis, 2018, 36(4): 380-387. (in Chinese)
	(江莉, 张耀光, 蔡黎, 等. 疟原虫种属同检多重PCR方法的建立和应用[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(4): 380-387.)
[7]	Jiang L, Cai L, Zhang YG, et al. Chimeric primer multiplex PCR molecular detection kit for co-detection of genus- and species-specific malaria and its detection method[P]. China: ZL201610181822.3, 2019-05-24. (in Chinese)
	(江莉, 蔡黎, 张耀光, 等. 疟疾种属同检的嵌合引物多重PCR分子检测试剂盒及其检测方法[P]. 中国: ZL 201610181822.3, 2019-05-24.)
[8]	Zhang L, Xia ZG. Epidemiological characteristics of malaria in China, 2023[J]. Chin J Parasitol Parasit Dis, 2024, 42(2): 135-139. (in Chinese)
	(张丽, 夏志贵. 2023年全国疟疾疫情特征分析[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(2): 135-139.) doi: 10.12140/j.issn.1000-7423.2024.02.001
[9]	Zhang YG, Jiang L, Wang ZY, et al. Analysis of the causes of misdiagnosis of seven imported malaria cases in Shanghai from 2020 to 2021[J]. Chin J Parasitol Parasit Dis, 2023, 41(1): 68-74. (in Chinese)
	(张耀光, 江莉, 王真瑜, 等. 2020—2021年上海市7例输入性疟疾病例误判原因分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(1): 68-74.) doi: 10.12140/j.issn.1000-7423.2023.01.010
[10]	Zhang YG, Jiang L, Wang ZY, et al. Laboratory diagnosis of two misdiagnosed imported Plasmodium ovale malaria cases in Shanghai[J]. Chin J Parasitol Parasit Dis, 2021, 39(4): 553-556. (in Chinese)
	(张耀光, 江莉, 王真瑜, 等. 上海市2例输入性卵形疟误诊病例的实验室诊断分析[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(4): 553-556.) doi: 10.12140/j.issn.1000-7423.2021.04.022
[11]	Zhu M, Wu HY, Zhang CG, et al. Epidemiological analysis of imported malaria reported in Shanghai, 2016-2019[J]. China Trop Med, 2021, 21(1): 55-59, 69. (in Chinese)
	(朱民, 吴寰宇, 张宸罡, 等. 上海市2016—2019年输入性疟疾流行特征[J]. 中国热带医学, 2021, 21(1): 55-59, 69.) doi: 10.13604/j.cnki.46-1064/r.2021.01.13

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