Study on early warning of high risk environment of Schistosoma japonicum infection by quantitative real-time PCR

doi:10.12140/j.issn.1000-7423.2023.04.018

Abstract

Abstract:

To rapidly monitor and warn high-risk environments with Schistosoma japonicum, detecting S. japonicum DNA in the visceral tissues of sentinel rats at the early stage of infection by quantitative real-time PCR (qRT-PCR). 20 infected Oncomelania hupensis were placed in 25 ℃ warm water to release cercariae. The rats (KM species) were divided into three groups randomly, i.e. Group A with natural cercarial infection, Group B with quantitative cercarial infection and Group C without cercarial infection as a negative control group. In Group A, 6 rats were transferred into the cage with the water with cercariae for 1 h, which imitate the natural infection field. 36 rats in Group B were infected with cercariae (40 ± 5 per mouse) via the abdominal skin route. 9 rats in Group C were not infected with cercariae. The rats in Group A were anatomized randomly on day 1, 2 and 5 after infection. The rats in Group B were anatomized randomly on day 1, 2, 3, 5, 7 and 14 after infection, respectively. The rats in Group C were randomly selected to anatomized synchronously with the Group A and B. The tissues of abdominal skin, heart, lung and liver of each rat were collected after the dissection. Then the genomic DNA of the total collected tissues was extracted and tested for schistosome DNA through qRT-PCR. The Ct values were recorded to determine the infection rates in each group, especially for Group B, in which each rat's DNA Ct value of the lung tissue needed to record at different time period. As the qRT-PCR results showed, schistosome DNA in Group A was detected in lung on day 1, 2 and 5 after infection, and in liver on day 5 after infection. While in group B, the schistosome DNA was detected in the lung tissue on day 1, 2, 3, 5, 7 and 14 after infection, and later in the liver tissue on day 2, 3, 5, 7 and 14. It showed that the schistosome DNA in lung in group B can be detected from the 2nd to the 5th day after infection. The results suggested that the detection of schistosome DNA in the lung tissue by qRT-PCR at the early stage of sentinel rat infection has the value for early monitoring and warning of the high-risk environment with schistosome infection.

Key words: Schistosoma japonicum, Sentinel rat, Early warning, High-risk environment, Quantitative real-time PCR

CLC Number:

R532.21

LAN Weiming, XU Hui, XU Yin, QIU Tingting, XIE Shuying, DENG Fenglin, HU Shaoliang, LIU Huan, GUO Jiagang, ZENG Xiaojun. Study on early warning of high risk environment of Schistosoma japonicum infection by quantitative real-time PCR[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 502-505.

Figures/Tables 1

References 20

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