Screening of cDNA Library of Schistosoma japonicum with Sera from Rabbits Vaccinated with Ultraviolet-attenuated Schistosomula

Abstract

Abstract: Objective To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae. Methods Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schitosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced. Results Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively. Conclusion Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.

Key words: Schistosoma japonicum, ultraviolet rays, DNA sequence analysis

LIXiao-hong;LIUShu-xian;SONGGuang-cheng;XUYu-xin;CAOJian-ping;CHENJia-xu. Screening of cDNA Library of Schistosoma japonicum with Sera from Rabbits Vaccinated with Ultraviolet-attenuated Schistosomula[J]. , 2004, 22(2): 4-82.

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Screening of cDNA Library of Schistosoma japonicum with Sera from Rabbits Vaccinated with Ultraviolet-attenuated Schistosomula

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