Expression of Proteinase Cathepsin L1 Gene of Schistosoma japonicum in Escherichia coli

Abstract

Abstract: Objective To express the proteinase cathepsin L1 gene of Schistosoma japonicum (SjCL1) in Escherichia coli JM109 cells. Methods The SjCL1 gene was amplified from the recombinant plasmid pcDNA3-SjCL1 by PCR. The gene was cloned into a prokaryotic expression vector pGEX-4T-1 to construct a recombinant plasmid pGEX-SjCL1. The E.coli JM109 cells were transformed with the recombinant plasmid pGEX-SjCL1 and the transformants were induced by IPTG to express the recombinant protein, the target protein was then identified by SDS-PAGE and Western blotting. Results A 1 kb length PCR product was obtained and a recombinant plasmid pGEX-SjCL1was constructed. The expression product was detected by SDS-PAGE and Western blotting and an expression band about 62 000 was found. Conclusion The SjCL1 gene is effectively expressed in the E. coli JM109 cells.

Key words: Schistosoma japonicum, cathepsins, gene expression

YIBing;LIZhuo-ya;HEAi;ZHENGXiao-ying;ZHENGBin;ZHOUHao-jie;WANGYi;ZHANGRui-lin;YUNan;ZHANXi-mei. Expression of Proteinase Cathepsin L1 Gene of Schistosoma japonicum in Escherichia coli[J]. , 2004, 22(2): 3-79.

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Expression of Proteinase Cathepsin L1 Gene of Schistosoma japonicum in Escherichia coli

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