FINDING OF NEW FCC1/HN ANTIGENIC EXPRESSED SEQUENCE TAG(ESTs) OF PLASMODIUM FALCIPARUM

Abstract

Abstract: 　AIM: To sequence the strong positive clones obtained by immuno-screening of Plasmodium falciparum FCC1/HN λgt11 cDNA expression library, and to elucidate the antigenic expressed sequence tags through sequencing the cDNA insert of these positive clones, and new antigenic ESTs could serve as a resource to pursue their corresponding antigen genes. METHODS: cDNA inserts of positive λgt11 phage clones were amplified by PCR. The PCR products, after purification, were cloned into the M13 mp18 sequencing vector. Single-stranded M13 DNA was prepared and sequenced. Then the acquired sequences were compared in homologies with EMBL/GenBank database on the PC/GENE software system and searched in NCBI (National Center for Biotechnology Information) GenBank using BLAST (Basic Local Alignment Search Tool) commond. RESULTS: Sequence C03 was part of the known P.falciparum antigenic heat shock protein 70 (Pfhsp70) gene, while the other 5 sequences were new P.falciparum antigenic expressed sequence tags (ESTs). CONCLUSION: The 5 new antigenic ESTs generated could serve as the breaking through points in our efforts to find out new P.falciparum antigen genes.

Key words: Plasmodium falciparum, antigen, cDNA, expressed sequence tags

YANZhonghe;XIEYi;LIMing;WANGPing;WANGYanni;BIHuixiang;LIYingjie. FINDING OF NEW FCC1/HN ANTIGENIC EXPRESSED SEQUENCE TAG(ESTs) OF PLASMODIUM FALCIPARUM[J]. , 1999, 17(6): 12-366.

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FINDING OF NEW FCC1/HN ANTIGENIC EXPRESSED SEQUENCE TAG(ESTs) OF PLASMODIUM FALCIPARUM

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