Abstract: A specific DNA fragment isolated from Plasmodium falciparum FCC1/HN isolate has been cloned in Bam H1 site of pUC18 and first partially sequenced by Sangers method.The results show∶G+C percent of DNA sequence is 26.8%,and the cloned DNA has many restriciton sites which are conventional for subcloning.The authors suggest that this clone and sequence may be used as a guide for developing a DNA probe or PCR primers.