Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang

doi:10.12140/j.issn.1000-7423.2021.03.006

Abstract

Abstract:

Objective To investigate the infection and distribution of Echinococcus multilocularis in small rodents, the intermediate hosts, in Chabuchaer County, Xinjiang, and to identify the intermediate host species of the parasite, providing scientific basis for formulating local control measures. Methods Small rodents were captured with plate clamps and bow clips in four selected investigation sites, including Jiagasitai town, Qiongbole town, Sishi and Dulata Port. Suspected infection in liver tissues was confirmed by HE staining and PCR amplification. The PCR products were sequenced. The species of small rodents were identified according to their physical appearance and skull morphology. Theχ² test was used to compare the infection rate in different regions and in different rodent populations. Results A total of 595 small rodents were captured, which belonged to 7 species, including 464 Microtus spp., 84 Apodemus sylvaticus, 14 Meriones tamariscinus Pallas, 14 Rhombomys sopimus Pallas, 8 Ellobius trancrei, 6 Cricetulus migratorius Pallas and 5 Meriones erythrourus Pallas. In 67 liver samples of suspected infection, lesions of E. multilocularis metacestodes were observed. HE staining showed that the protoscoleces of E. multilocularis metacestodes were visible in the lesion tissues. PCR amplification and sequence alignment showed that all the 67 small rodents were infected with metacestodes of E. multilocularis, with a total infection rate of 11.3%. All the seven species of rodents were infected, with infection rates of 12.8% (57/464), 3.6% (3/84), 1/14, 2/14, 2/8, 1/5 and 1/6 for Microtus spp, A. sylvaticus, M. tamariscinus Pallas, R. sopimus Pallas, E. trancrei, M. serythrourus Pallas and C. migratorius Pallas, respectively. Chi-square analysis showed that there was a significant difference in the infection rate between Microtus spp. and A. sylvaticus (P < 0.05). The infection rate of Microtus spp. did not significantly differ from that of the other species (P > 0.05). Of the 4 investigation sites, the Qiongbole town showed the highest infection rate (12.3%, 49/398). Conclusion The small rodents captured in Chabuchaer County were mainly Microtus spp. and A. sylvaticus, with the former species showing the highest infection rate of metacestodes.

Key words: Metacestodes of Echinococcus multilocularis, Echinococcus multilocularis, Small rodent, Microtus spp., Chabuchaer County, Xinjiang

CLC Number:

R532.32

GUO Bao-ping, GUO Gang, ZHANG Li, XIANG Jing-jing, WANG Xiao-ping, REN Yuan, QI Wen-jing, ZHANG Hui, LI Jun, ZHANG Wen-bao, WANG Hai-yan. Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(3): 327-332.

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References 24

[1]	Deplazes P, Rinaldi L, Alvarez Rojas CA, et al. Global distribution of alveolar and cystic echinococcosis[J]. Adv Parasitol, 2017,95:315-493.
[2]	Zhang MY, Wang LY, Guan YY, et al. Meta-analysis on severity of hydatidosis[J]. Chin J Parasitol Parasit Dis, 2018,36(2):l56-160. (in Chinese)
[2]	( 张梦媛, 王立英, 官亚宜, 等. 棘球蚴病严重程度的Meta分析[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(2):156-160.)
[3]	Torgerson PR, Keller K, Magnotta M, et al. The global burden of alveolar echinococcosis[J]. PLoS Negl Trop Dis, 2010,4(6):e722.
[4]	Wen H, Vuitton L, Tuxun T, et al. Echinococcosis: advances in the 21st century[J]. Clin Microbiol Rev, 2019,32(2):e00075-e00018.
[5]	Zhang WB, Zhang ZZ, Shi BX, et al. Vaccination of dogs against Echinococcus granulosus the cause of cystic hydatid disease in humans[J]. J Infect Dis, 2006,194(7):966-974.
[6]	Giraudoux P, Raoul F, Pleydell D, et al. Drivers of Echinococcus multilocularis transmission in China: small mammal diversity, landscape or climate?[J]. PLoS Negl Trop Dis, 2013,7(3):e2045.
[7]	Lin YG, Hong LX. The biology and geographical distribution of Echinococcus multilocularis infection in China[J]. Endem Dis Bull, 1991,6(2):117-127. (in Chinese)
[7]	( 林宇光, 洪凌仙. 我国多房棘球绦虫病的病原生物学及其在我国的地理分布[J]. 疾病预防控制通报, 1991,6(2):117-127.)
[8]	Gesy K, Hill JE, Schwantje H, et al. Establishment of a European-type strain of Echinococcus multilocularis in Canadian wildlife[J]. Parasitology, 2013,140(9):1133-1137.
[9]	Grishailla I. Observation on the curative effect of albendazole on hepatic hydatid disease[J]. Chin Pract J Rural Dr, 2016,23(6):57-58. (in Chinese)
[9]	( 古丽沙依拉·英斯.. 阿苯达唑治疗肝包虫病疗效观察[J]. 中国实用乡村医生杂志, 2016,23(6):57-58.)
[10]	Wang Z, Wang X, Liu X. Echinococcosis in China, a review of the epidemiology of Echinococcus spp[J]. Ecohealth, 2008,5(2):115-126.
[11]	Tang CT, Quian YC, Kang YM, et al. Study on the ecological distribution of alveolar Echinococcus in Hulunbeier Pasture of Inner Mongolia, China[J]. Parasitology, 2004,128(Pt 2):187-194.
[12]	Wang C, He H, Li M, et al. Parasite species associated with wild plateau pika (Ochotona curzoniae) in southeastern Qinghai Province, China[J]. J Wildl Dis, 2009,45(2):288-294.
[13]	Wang W, Wu Y, Wu JG, et al. The occurence ofEchinococcus multilocularis leukart, 1863 in fox and wolf in Tacheng district, Xinjiang[J]. Endem Dis Bull, 1989,4(2):8-11. (in Chinese)
[13]	( 王伟, 吴勇, 吴季高, 等. 新疆塔城地区多房棘球绦虫调查: 国内狼体多房棘球绦虫新记录[J]. 地方病通报, 1989,4(2):8-11.)
[14]	Holt DW, Hanns C, O’Hara T, et al. New distribution records of Echinococcus multilocularis in the brown lemming from Barrow, Alaska, USA[J]. J Wildl Dis, 2005,41(1):257-259.
[15]	Wang SY, Yang GY. Annals of Xinjiang rodents[M]. Urumqi: Xinjiang People’s Publishing House, 1983: 8-28. (in Chinese)
[15]	( 王思博, 杨赣源. 新疆啮齿动物志 [M]. 乌鲁木齐: 新疆人民出版社, 1983: 8-28.)
[16]	Pleydell DR, Yang YR, Danson FM, et al. Landscape composition and spatial prediction of alveolar echinococcosis in southern Ningxia, China[J]. PLoS Negl Trop Dis, 2008,2(9):e287.
[17]	Wu C, Zhang W, Ran B, et al. Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in Western China[J]. Parasit Vectors, 2017,10(1):265.
[18]	Giraudoux P, Pleydell D, Raoul F, et al. Transmission ecology of Echinococcus multilocularis: what are the ranges of parasite stability among various host communities in China?[J]. Parasitol Int, 2006,55:S237-S246.
[19]	Wen H, Zhang YL, Bart J, et al. Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog[J]. Chin J Parasitol Parasit Dis, 2006,24(1):10-13. (in Chinese)
[19]	( 温浩, 张亚楼, Bart J, 等. 犬体内细粒棘球绦虫和多房棘球绦虫的混合感染[J]. 中国寄生虫学与寄生虫病杂志, 2006,24(1):10-13.)
[20]	Wang DM, He RF, Gong S, et al. Prevalence of echinococcosis in Nyingchi City[J]. Chin J Parasitol Parasit Dis, 2018,36(1):75-79. (in Chinese)
[20]	( 王栋民, 何瑞峰, 贡桑曲珍, 等. 林芝市棘球蚴病流行情况[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(1):75-79.)
[21]	Van Kesteren F, Mastin A, Mytynova B, et al. Dog ownership, dog behaviour and transmission of Echinococcus spp. in the Alay Valley, southern Kyrgyzstan[J]. Parasitology, 2013,140(13):1674-1684.
[22]	Liccioli S, Giraudoux P, Deplazes P, et al. Wilderness in the ‘City’ revisited: different urbes shape transmission of Echinococcus multilocularis by altering predator and prey communities[J]. Trends Parasitol, 2015,31(7):297-305.
[23]	Guo BP. Study on correlation between pathogenic differences and mitochondrial genetic markers in Echinococcus multilocularis[D]. Shihezi, China: Shihezi University, 2019: 2-6. (in Chinese)
[23]	( 郭宝平. 多房棘球绦虫致病差异与线粒体遗传标志相关性的研究[D]. 石河子: 石河子大学, 2019: 2-6.)
[24]	Raoul F, Deplazes P, Rieffel D, et al. Predator dietary response to prey density variation and consequences for cestode transmission[J]. Oecologia, 2010,164(1):129-139.

种属Species	加尕斯台乡 Jiagasitai Town		琼博乐乡 Qiongbole Town		四师Sishi		都拉塔口岸Dulata port		合计Total
种属Species	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)
普通田鼠 Microtus spp.	71	8（11.3）	366	46（13.9）	19	2（2/19）	8	1（1/8）	464	57（12.8）
坦氏鼹形田鼠 E. trancrei	0	0	8	2（2/8）	0	0	0	0	8	2（2/8）
小林姬鼠 A. sylvaticus	23	1（4.4）	24	1（4.2）	17	1（1/17）	20	0	84	3（3.6）
柽柳沙鼠M. tamariscinus Pallas	0	/	0	/	7	0	7	1（1/7）	14	1（1/14）
大沙鼠 R. sopimus Pallas	0	/	0	/	7	1（1/7）	7	1（1/7）	14	2（2/14）
红尾沙鼠 M. erythrourus Palla	0	/	0	/	0	/	5	1（1/5）	5	1（1/5）
灰仓鼠 C. migratorius Pallas	0	/	0	/	0	/	6	1（1/6）	6	1（1/6）
合计Total	94	9（9.6）	398	49（12.3）	50	4（8.0）	53	5（9.4）	595	67（11.3）