Ultrasound and pathological manifestations of lesions in SD rats with hepatic Echinococcus multilocularis infection established by different methods

doi:10.12140/j.issn.1000-7423.2022.04.021

Abstract

Abstract:

100 male SD rats were randomly divided into the liver puncture group and the portal vein injection group, with 50 rats in each group. The SD rat hepatic Echinococcus multilocular infection models were established by open liver puncture and portal vein injection. The survival rates of the two groups were compared after infection. The infection rate in the two groups was calculated by ultrasonic examination and open examination 4 months after infection. The ultrasonic manifestations of lesions in the two groups were compared. Hematoxylin-eosin (HE) staining was used to observe the liver lesions of the two groups. The results showed that the postoperative survival rate of rats in the liver puncture group was 96.0% (48/50), which was higher than 84.0% (42/50) in the portal vein group (χ² = 4.000, P < 0.05). The infection rate in the liver puncture group was 56.3% (27/48), and there was no significant difference compared to that of the portal vein group (61.9%, 26/42) (χ² = 0.296, P > 0.05). Ultrasound results showed that of the 27 rats successfully inoculated in the liver puncture group, 4 had multiple lesions, and the remaining 21 rats showed single intrahepatic lesions. The 26 rats successfully inoculated in the portal vein group showed single E. multilocular infection (P < 0.05). The lesions in the liver puncture group were all located in the left lobe of the liver. The lesions in the portal vein group were all located in the right lobe of the liver (P < 0.05). The liver lesions of the 12 rats in the liver puncture group were solid lesions with uniform internal echoes, 7 were solid lesions with uneven echoes and calcifications, and 8 were non-uniform echoes with internal echoes of different sizes, and mixed lesions of echogenic vesicles. The liver lesions of 6 rats in the portal vein group showed solid lesions with uniform internal echo, 16 rats showed mixed lesions with uneven echo and internal vesicles of different sizes, and 4 showed mixed lesions with heterogeneous internal echoes accompanied by calcification and vesicles. The difference between the two groups was statistically significant (P < 0.01). The average maximum diameter of liver lesions in the liver puncture group was (5.86 ± 2.69) mm, which was smaller than that in the portal vein group (11.69 ± 5.94) mm (t = -4.578, P < 0.01). The results of HE staining showed that all the lesions were infected by E. multilocularis. There were obvious germinal structures in the liver of rats in the liver puncture group and portal vein group, and there were varying degrees of inflammatory cell infiltration and proliferative fibrous connective tissue around the lesions. It is suggested that both methods can establish the rat model of hepatic E. multilocularis infection, and there are differences in ultrasonic manifestations. The portal vein puncture injection method is more suitable for establishing the animal model of E. multilocular infection.

Key words: Echinococcus multilocularis, Hepatic alveolar echinococcosis, Animal models, Ultrasonography

CLC Number:

R532.1

WU Liang-liang, YANG Ling-fei, SONG Tao. Ultrasound and pathological manifestations of lesions in SD rats with hepatic Echinococcus multilocularis infection established by different methods[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(4): 549-552.

Figures/Tables 2

References 12

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