Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang

doi:10.12140/j.issn.1000-7423.2021.03.006

Abstract

Abstract:

Objective To investigate the infection and distribution of Echinococcus multilocularis in small rodents, the intermediate hosts, in Chabuchaer County, Xinjiang, and to identify the intermediate host species of the parasite, providing scientific basis for formulating local control measures. Methods Small rodents were captured with plate clamps and bow clips in four selected investigation sites, including Jiagasitai town, Qiongbole town, Sishi and Dulata Port. Suspected infection in liver tissues was confirmed by HE staining and PCR amplification. The PCR products were sequenced. The species of small rodents were identified according to their physical appearance and skull morphology. Theχ² test was used to compare the infection rate in different regions and in different rodent populations. Results A total of 595 small rodents were captured, which belonged to 7 species, including 464 Microtus spp., 84 Apodemus sylvaticus, 14 Meriones tamariscinus Pallas, 14 Rhombomys sopimus Pallas, 8 Ellobius trancrei, 6 Cricetulus migratorius Pallas and 5 Meriones erythrourus Pallas. In 67 liver samples of suspected infection, lesions of E. multilocularis metacestodes were observed. HE staining showed that the protoscoleces of E. multilocularis metacestodes were visible in the lesion tissues. PCR amplification and sequence alignment showed that all the 67 small rodents were infected with metacestodes of E. multilocularis, with a total infection rate of 11.3%. All the seven species of rodents were infected, with infection rates of 12.8% (57/464), 3.6% (3/84), 1/14, 2/14, 2/8, 1/5 and 1/6 for Microtus spp, A. sylvaticus, M. tamariscinus Pallas, R. sopimus Pallas, E. trancrei, M. serythrourus Pallas and C. migratorius Pallas, respectively. Chi-square analysis showed that there was a significant difference in the infection rate between Microtus spp. and A. sylvaticus (P < 0.05). The infection rate of Microtus spp. did not significantly differ from that of the other species (P > 0.05). Of the 4 investigation sites, the Qiongbole town showed the highest infection rate (12.3%, 49/398). Conclusion The small rodents captured in Chabuchaer County were mainly Microtus spp. and A. sylvaticus, with the former species showing the highest infection rate of metacestodes.

Key words: Metacestodes of Echinococcus multilocularis, Echinococcus multilocularis, Small rodent, Microtus spp., Chabuchaer County, Xinjiang

CLC Number:

R532.32

GUO Bao-ping, GUO Gang, ZHANG Li, XIANG Jing-jing, WANG Xiao-ping, REN Yuan, QI Wen-jing, ZHANG Hui, LI Jun, ZHANG Wen-bao, WANG Hai-yan. Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(3): 327-332.

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种属Species	加尕斯台乡 Jiagasitai Town		琼博乐乡 Qiongbole Town		四师Sishi		都拉塔口岸Dulata port		合计Total
种属Species	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)
普通田鼠 Microtus spp.	71	8（11.3）	366	46（13.9）	19	2（2/19）	8	1（1/8）	464	57（12.8）
坦氏鼹形田鼠 E. trancrei	0	0	8	2（2/8）	0	0	0	0	8	2（2/8）
小林姬鼠 A. sylvaticus	23	1（4.4）	24	1（4.2）	17	1（1/17）	20	0	84	3（3.6）
柽柳沙鼠M. tamariscinus Pallas	0	/	0	/	7	0	7	1（1/7）	14	1（1/14）
大沙鼠 R. sopimus Pallas	0	/	0	/	7	1（1/7）	7	1（1/7）	14	2（2/14）
红尾沙鼠 M. erythrourus Palla	0	/	0	/	0	/	5	1（1/5）	5	1（1/5）
灰仓鼠 C. migratorius Pallas	0	/	0	/	0	/	6	1（1/6）	6	1（1/6）
合计Total	94	9（9.6）	398	49（12.3）	50	4（8.0）	53	5（9.4）	595	67（11.3）