›› 1997, Vol. 15 ›› Issue (3): 13-169.

• 论著 • Previous Articles     Next Articles

PRELIMINARY STUDY ON CHARACTERIZATION OF THE LEISHMANLA DONOVANI GENE ENCODING 39k Da ANTIGEN

Jing Baoqian1,Hu Xiaosu2,Liu Hongbin2,Cheng Jianpin2   

  1. 1　Department of Microbiology, North Sichuan Medical College, Nanchong　6370002　Laboratory of Parasitology, West China University of Medical Sciences, Chengdu　610044
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-06-30 Published:1997-06-30

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Abstract:

AIM: To characterize the L. donovani gene encoding 39 kDa antigen. METHODS: The recombinant phage DNA fragment expressing 39 kDa antigen of L. donovani was subcloned in to pUC18 vector and further analyzed by restriction endonuclease digestion. RESULTS: The size of the inserted fragment was nearly 1.3 kb with a single site recognized by Kpn I, Xba I, HincⅡ and Pst I respectively at the 3’-end of the fragment. CONCLUSION: The inserted fragment might be a single copy containing only one reading frame and one non-encoding region.

Key words: Leishmania donovani, antigen, gene.