Abstract:

　Objective To analyze soluble antigens of Trichomonas vaginalis. \ Methods\ Soluble antigens of the parasite from a patient suffering from trichomonad vaginitis were analyzed by SDS-PAGE, immunoblotting and two-dimensional gel electrophoresis. \ Results\ A total of 26 distinct protein bands were demonstrated by using 10% resolution gel. Nine of them were main bands, eight with MWs 15-62 kDa, one with MW 97 kDa. By immunoblotting the specific anti-T. vaginalis antibodies raised in rabbit recognized 24 protein bands with 8 main bands in them. Two-dimensional gel electrophoresis revealed up to 43 individual trichomonad polypeptide spots, among which, 9 were main ones. The pI and MWs of these spots were 3.65-5.84 and 27->100 kDa respectively. \ \{Conclusion\ Eight\} protein bands out of 26 soluble antigen bands of the parasite showed high immunogenicity. There were 9 main polypeptide spots in 43 polypeptide spots of the parasite.

Key words: Trichomonas vaginalis, antigen, SDS-PAGE, two-dimensional gel electrophoresis.