›› 2007, Vol. 25 ›› Issue (1): 2-11.

• 论著 • Previous Articles     Next Articles

Cloning and Fusion Expression of Adenosine Deaminase of Schistosoma japonicum

YANG Zhong1;XU Bin2;WANG Wei1,2;FENG Zheng2;WEI Dong-zhi1;HU Wei2   

  1. 1 State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China Uiniversity of Science and Technology, Shanghai 200237, China; 2 National Institute of Parasitic Dis?鄄eases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schis?鄄tosomiasis and Filariasis, Shanghai 200025, China； 3 National Human Genome Center at Shanghai, Shanghai 201323, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-28 Published:2007-02-28
  • Contact: HU Wei

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Abstract: Objective To clone and express a new protein adenosine deaminase of Schistosoma japonicum (SjADA). Method Specific primers were designed according to the EST sequence and used for amplification of the encoding sequence from the cDNA clone containing SjADA. The gene was sub-cloned into pET32 plasmid and expressed in E.coli BL31(DE) pLys strain and the recombinant proteins were purified by chelating sepharose FF. The structure and phylifunctions of this protein were analyzed by MrBayes and GeneAtlas. Result The full length sequence of SjADA gene was obtained and the recombinant protein was cloned, expressed and purified successfully. The bioinformatics analysis showed that the identity of SjADA and SmADA gene sequence was only 25% and they belonged to different subfamily. The structure of SjADA had 41% identity with it’s PDB template 1A4M. Conclusion The recombinant protein of SjADA has been obtained. The purine salvage pathway of S. japonicum may be different with that of S.mansoni.

Key words: Schistosoma japonicum, Purine metabolism, ADA