›› 2006, Vol. 24 ›› Issue (5): 16-386.

• 研究简报 • Previous Articles     Next Articles

Construction of Sjcb2 DNA Vaccine and its Expression in HeLa Cells

HU Yong-xuan1，2，XIAO Jian-hua1，HUANG Jia-fang1，3，YANG Qiu-lin1   

  1. 1 Institute of Pathogenic Biology, Nanhua University, Hengyang 421001, China；2 Institute of Microbiology and Immunology, Xiangnan College, Chenzhou 423000, China；3 Aerospace Engineering Company Hospital, Beijing 100800, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-10-30 Published:2006-10-30

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Abstract: A recombinant plasmid containing cathepsin B endopeptidase of Schistosoma japonicum （Sjcb2） was constructed, indentified by PCR, restrictive enzyme, digestion and DNA sequencing, and expressed into mammalian cells. Immunochemistry examination showed that the Sjcb2 gene can be expressed in the eukaryotic system, providing a basis for the development of schistosome DNA vaccine.

Key words: Schistosoma japonicum, Cathepsin B Endopeptidase, DNA vaccine, Eukaryotic expression