Detection of Biliverdin Reductase Activity of Schistosoma japonicum

Abstract

Abstract: 　Objective To explore the presence of biliverdin reductase (BR) activity in adult worms of Schistosoma japonicum in vitro. Methods The soluble fraction was isolated from homogenates of adult worms of S. japonicum, and incubated with biliverdin under different pH conditions and buffers. The time dependency of this enzymatic reaction was also detected. ResultsThe soluble fraction of the homogenates of adult worms could degrade biliverdin in vitro, the BR activity was 43.30 nmol/(mg·min), with its optimal condition at pH 8.7. The rate of reaction peaked at 15 min of incubation for the BR activity. Conclusion The presence of BR activity in adult worms of S. japonicum was firstly demonstrated.

Key words: Schistosoma japonicum, biliverdin, bilirubin, oxidoreductases

LIUWen-qi;LIYong-long. Detection of Biliverdin Reductase Activity of Schistosoma japonicum[J]. , 2004, 22(2): 12-108.

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Detection of Biliverdin Reductase Activity of Schistosoma japonicum

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