Abstract: 　Objective To prepare and characterize the polyclonal and monoclonal antibodies
McAbs against Schis- tosoma japonicum(Sj) signaling protein 14-3-3. Methods Recombinant Sj14-3-3 protein was expressed and the gel slices were lyophilized. Rabbits were immunized with the ground gel powder to prepare polyclonal antibodies against Sj14-3-3. BALB/c mice were immunized with recombinant Sj14-3-3 purified by electroelution. The anti-rSj14-3-3 monoclonal anti- body was obtained by using hybridoma technique. Characterization of the antibodies was performed by enzyme linked im- munosorbent assay (ELISA) and Western blotting analysis. Results Purified recombinant Sj14-3-3 protein was ob- tained. The titers of immune sera from the animals were 1: 8-1: 64. The monoclonal antibody(McAb) against 14-3-3 antigen was obtained and its subclass was found to be IgG1. The McAb reacted strongly and specifically with the expressed Sj14-3-3 protein. Conclusion The anti-14-3-3 sera were obtained and the hybridoma cell lines which secreted stable monoclonal antibodies against Sj14-3-3 were established, which provides a new approach to study the role of Sj14-3-3 in the signal transduction of S. japonicum.

Key words: Schistosoma japonicum, Sj14-3-3 protein, monoclonal antibody