›› 2003, Vol. 21 ›› Issue (4): 7-220.

• 论著 • Previous Articles     Next Articles

Study on the Effect of H_2O_2 Against Acanthamoeba in vitro

ZHAO Qun-fei,GAO Xue-liang,QIAN Min
  

  1. State Key Laboratory of Bio-Orgnic and Natural Product chemistry,Shanghai Institute of Orgnic Chemistry,Chinese Academy of Science,Shanghai 200032 School of Life Science,East China Normal University,Shanghai 200062
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-30 Published:2003-08-30

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Abstract: 　Objective To detect the effect of H_2O_2 on Acanthamoeba spp.. Methods By Wright's stain, quanti- tative culture, MTT assay and lactate dehydrogenase
LDH assessment, the in fluence of H_2O_2 on the morphological fea- ture, proliferation speed and the survival rate of Acanthamoeba was tested. Results At low concentration of 0. 125%, H_2O_2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H_2O_2 can directly de- stroy Acanthamoeba trophozoites. Conclusion H_2O_2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.

Key words: Acanthamoeba lugdunensis-A.quina, H_2O_2