Abstract: 　[Objective] To understand the components of the crude antigens of Echinococcus multilocularis protoscoleces. [Methods] Isoelectric focusing was carried out to fractionate the protein components of E.multilocularis protoscoleces. The individual fractions were collected and subjected to SDS-PAGE, the gels were analyzed by a gel analysis system. [Results] The pI values of individual fractions varied from 4.0 to 8.5, while the molecule weights of main protein bands were 72/73、62/63、42/44、35/36、34 and 21/22 kDa. [Conclusion] An alternative separation method of crude antigens of Echinococcus multilocularis was established. \;

Key words: Isoelectric focusing, Echinococcus multilocularis, protoscolex antigen