Abstract: 　Objective]To clone and express Cysticercus Cellulosae antigen cC1 in E.coli . [Methods] cC1cDNA fragment was cloned to BamHI and PstI sites of pGEM 3Z vector.After alteration of the restriction sites,the fragment was cloned to EcoRI and XhoI sites of pGEX 5T with a synthetic linker to construct recombinant expression vector pGEX 5T cC1. [Results] The clone produced the largest yield of cC1 protein expression when incubated in 2YT culture medium for 3 h or induced by IPTG for 6 h.Detected by scanning optical densitometry, cC1 constituted 57% of the total bacterial proteins. Western blotting analysis revealed that the GST cC1 fusion protein exhibited a specific reactive band.[Conclusion] High level expression of Cysticercus cellulosae antigen cC1 was obtained in E.coli .

Key words: Cysticercosis, antigen, gene expression.