›› 1998, Vol. 16 ›› Issue (5): 357-359.

• 论著 • Previous Articles     Next Articles

STUDIES ON DYNAMIC CHANGES OF SOD AND MDA IN CULTURED CELLS FROM SCHISTOSOMA JAPONICUM SCHISTOSOMULA

Jiang Mingsen; Chen Xiaobei* ; Dong Huifen; Yang Mingyi; Ni Yonghui; Zhou Shulong   

  1. Department of Medical Parasitology and Research Laboratory of Schistosomiasis; Hubei Medical University; Wuhan 430071  *Department of Infectious Disease; The First Affiliated Hospitol of HubeiMedical University ,Wuhan 430060
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-10-31 Published:1998-10-31

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Abstract:

AIM:To observe the dynamic changes of superoxide dismutase(SOD) and

maldondialdehyde(MDA) in cultured cells from Schistosoma japonicum schistosomula and to explore

the degeneration process of cultured cells. METHODS: Pyrogallol method and thiobarbituric acid

method were used to determinate the levels of SOD and MDA , respectively,in the cells cultured

for 0-54 days. RESULTS: SOD in the cells reached a peak level on d12 of the early stage of

culture and then decrease. The MDA levels in the cultures cells ascended gradually, and had two peaks on d18 and d48, during the period of culture. CONCLUSION: The cultured cells from S. japonicum schistosomula degenerated progressively.

Key words: Schistosoma japonicum, schistosomula, cultured cells, SOD, MDA