IMMUNODIAGNOSIS OF CYSTICERCOSIS BY SIMPLIFIED WESTERN BLOTTING USING RECOMBINANT  FUSION PROTEIN AS ANTIGEN

›› 1998, Vol. 16 ›› Issue (5): 360-363.

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IMMUNODIAGNOSIS OF CYSTICERCOSIS BY SIMPLIFIED WESTERN BLOTTING USING RECOMBINANT FUSION PROTEIN AS ANTIGEN

Peng Yucong¹ ;Chen Ruiwen ² ;Sun Shuhan ²; Dai Jianxin ²; Wang Junxia ¹

1 Department of Molecular Biology; Hebei Medical University; Shijiazhuang 050017 2 Department of Molecular Genetics; Second Military Medical University, Shang hai 200433

Received:1900-01-01 Revised:1900-01-01 Online:1998-10-31 Published:1998-10-31

Abstract

Abstract: AIM:To provide simple and useful method for the immunodiagosis of cysticercosis.METHODS:Fusion protein of β galactosidase Cysticercus cellulosae cDNA was
analysed with simplified Western blot.RESULTS:The positive rate was 93.5% when detecting 124
cases of human cysticercosis sera with four clones (cC1,cC2,cP1 and cH1) and 100% when detecting
38 cases of porcine cysticercosis sera with three clones (cC1,cC2 and cP1),being higher than those using monoclone fusion protein alone. Moreover, the fusion proteins did not react with other parastitosis sera. CONCLUSION: Simplified Western blot analysis using recombinant fusion protein as antigen was highly sensitive and specific and easy to be operated.

Key words: Cysticercosis, fusion protein, immunodiagnosis, simplified Western blot

PengYucong;ChenRuiwen;SunShuhan;DaiJianxin;WangJunxia. IMMUNODIAGNOSIS OF CYSTICERCOSIS BY SIMPLIFIED WESTERN BLOTTING USING RECOMBINANT FUSION PROTEIN AS ANTIGEN[J]. , 1998, 16(5): 360-363.

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IMMUNODIAGNOSIS OF CYSTICERCOSIS BY SIMPLIFIED WESTERN BLOTTING USING RECOMBINANT FUSION PROTEIN AS ANTIGEN

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