›› 1997, Vol. 15 ›› Issue (6): 378-381.

• 论著 • Previous Articles     Next Articles

PARTIAL SEQUENCE OF NADH DEHYDROGENASE 1 AND CYTOCHROME C OXIDASE 1 GENE OF SCHISTOSOMA JAPONICUM

Qiu Chiping1; Zhang Lihua2; E.Sorensen2; D.P.Mc Manus2   

  1. 1 Institute of Parasitic Diseases, Chinese Academy of Prevantive Medicine, Shanghai 200025 2 Molecular Helminthology Laboratory, Tropical Health Program, The Queensland Institute of Medical Research, Australia
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

PDF (PC)

Abstract: AIM:To determine and compare NADH dehydrogenase1( NADH1) and cytochrome C oxidase1( Cytc1) partial
gene sequences of Zhejiang and Anhui isolates of Schistosoma japonicum. METHODS: NADH1 and Cytc1 genes were amplified by PCR using special primers corresponding with 44 1and 370 bp of the genomic DNAs, and subsequently sequenced. RESULTS:NADH1 and Cytc1 gene sequences were obtained from the two isolates. CONCLUSION: The partial sequences of the NADH1 genes were essentially identical for both isolates with only one nucleotide difference (C/T ) at position 382. The Cytc1 sequences were virtually identical for the two isolates.

Key words: Schistosom a japonicum, NADH dehydrogenase1, cytochrome C oxidase1, PCR, sequence