Phylogenetic and sequence analysis of mitochondrial nad1 of Metorchis orientalis strains from different geographic regions

doi:10.12140/j.issn.1000-7423.2024.05.017

Abstract

Abstract:

To examine the genetic variation and phylogenetic relationship of nicotinamide adenine dinucleotide dehydrogenase subunits 1 (pnad1) of Metorchis orientalis from different geographic regions. The quail were infected with M. orientalis metacercaria isolated from Pseudorasbora parvain in Fujian Province and Jiangxi Province, respectively. Adult worms were collected after 30 days, and the mitochondrial genomic DNA was extracted from 3 adults in each region. The pnad1 was amplified from M. orientalis by PCR and sequenced. The obtained sequences were compared with the mitochondrial pnad1 sequences from Heilongjiang (GenBank: KY232061, KY232066, KY232071) and published sequences of the same genus using BioEdit software and the species-specific and inter-species differences were analyzed. The phylogenetic distance of different regions was calculated by MEGA 11.0 software, and the phylogenetic tree of opisthorchidae based on mitochondrial pnad1 sequence was constructed by the adjacency method. The results showed that 500-bp bands were amplified in the mitochondria of the M. orientalis from Fujian and Jiangxi, and the mitochondrial pnad1 gene was 523 bp. The results of sequence comparison showed that the intraspecific genetic variation rate of pnad1 sequence was 0-0.8%, and the interspecific genetic variation rate was 12.6%-13.9%. The constracted phylogenetic tree based on the pnad1 gene sequences showed that M. orientalis isolates from Fujian, Jiangxi and Heilongjiang were clustered together, M. xanthosomus (GenBank: KT740991) and M. bilis (GenBank: NC079698) were clustered in a branch. It was concluded that the mitochondrial pnad1 sequence is relatively conserved within species and varies greatly between species, which can be used as a marker for studying interspecific genetic variation.

Key words: Meotrchis orientalis, Mitochondrial DNA, nad1 gene, Sequence analyses, Phylogeny

CLC Number:

R383.29

CHEN Yunhong, XIE Xianliang, LI Yanrong, CHEN Baojian, LIN Yaoying, YU Wenwu, YU Haifeng. Phylogenetic and sequence analysis of mitochondrial nad1 of Metorchis orientalis strains from different geographic regions[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(5): 668-671.

Figures/Tables 3

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