Phylogenetic analysis of Angiostrongylus cantonensis from Achatina fulica based on COⅠ sequence

doi:10.12140/j.issn.1000-7423.2023.06.014

Abstract

Abstract:

The genetic evolution of Angiostrongylus cantonensis infected by the Achatina fulica in Fuzhou was analyzed, and the phylogenetic relationship between A. cantonensis isolates from 5 continents was discussed. The A. fulica were collected from the garbage dump, grass and vegetable fields near the residential areas of major parks in Fuzhou City. The A. cantonensis stage Ⅲ larvae were collected by homogenizing method and identified by morphology. The larval DNA was extracted, and the cytochrome C oxidase subunit Ⅰ (COⅠ) sequence was amplified by PCR. After sequencing, BLAST comparison was performed using the GenBank database. The phylogenetic tree was constructed by the neighbour-joining method using MEGA software with A. vasorum as an outgroup. A total of 103 A. fulica were collected, and 19 of them were infected with stage Ⅲ A. cantonensis larvae. The infection rate was 18.4%. A total of 1 081 A. cantonensis stage Ⅲ larvae were detected. A 450 bp band was amplified by PCR. BLAST results showed that the COⅠ sequence obtained in this study was 98.2% consistent with that of A. cantonensis (GenBank accession number: AB684364.1). Phylogenetic tree analysis showed that the sequences obtained in this study converged on an evolutionary clade with the sequences of the Fujian isolates. The isolates were further clustered with the North America isolates (GenBank accession number: MH069731.1), South America isolates (GenBank accession number: MW390969.1), Oceania isolates (GenBank accession number: KU532144.1) and Europe isolates (GenBank accession number: MN227185.1) into a branch. Therefore, the Fuzhou isolate still belongs to the Asian geographical strain, and the genetic differentiation is stable.

Key words: Achatina fulica, Angiostrongylus cantonensis, Cytochrome C oxidase subunit Ⅰ, Sequence analysis, Phylogeny

CLC Number:

R383.19

GAO Lanlin, XIE Hanguo, LIN Chenxin, JIANG Dianwei, CAI Wuwei, ZHENG Dan. Phylogenetic analysis of Angiostrongylus cantonensis from Achatina fulica based on COⅠ sequence[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 756-759.

Figures/Tables 3

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