›› 1997, Vol. 15 ›› Issue (6): 382-385.

• 论著 • Previous Articles     Next Articles

A PRELIMINARY STUDY ON DETECTING PLASMODIUM FALCIPARUM BASED ON PCR AMPLIFICATION OF 3~, -END FRAGMENT OF CSP GENE

Chen Zhihui2; Wu Shaoting1; Guan Weibin2; Gao Shitong1; L in Min1   

  1. 1 Shenzhen Sanitary and Epidemic Disease Prevention Station, Shenzhen 518020 2 Department of Parasitology, The Second Military Medical University, Shanghai 200433
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

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Abstract: AIM:To develop a new method based on polymerase chain reaction(PCR) for detecting Plasmodium
falciparum. METHODS:Using 2 oligonucleotide primers with P.falciparum specificity designed and synthesized by the
authors, a PCR was conducted to amplify the highly conserved region(245 bp) at 3 end of CSP gene. The specificity, sensitivity and stability of the detection system were investigated. RESULTS:A 245 bp fragment from 4 cultured P.falciparum strains and two falciparum malaria patients’ blood samples was amplified, while the extracted DNA from P. vivax, Leishmania donovani, Toxoplasma gondii, and the normal blood sample could not. The amplified DNA was confirmed by using the known CSP gene as template to amplify the same size DNA fragmen t. As little as DNA of 0.18 parasite was sufficient for a specific detection by the PCR assay. CONCLUSION: The results show that the PCR assay for the detection of P.falciparum is specific, sensitive and stable.

Key words: Plasmodium falciparum, circumsporozoite protein gene, polymerase chain reaction, diagnosis