›› 1997, Vol. 15 ›› Issue (3): 7-151.

• 论著 • Previous Articles     Next Articles

THE INHIBITORY EFFECT OF ANTI-MAC-1 MONOCLONAL ANTIBODIES ON THE INVASION OF LEISHMANIA DONOVANL

Xue Changgui, Yang Liuping, Cui Jing   

  1. Department of Parasitology, Henan Medical University, Zhengzhou 450052
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-06-30 Published:1997-06-30

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Abstract:

AIM: To study the effect of anti Mac-1monoclonal antibodies (McAbs), M1/17 and M18/2, on the invasion of Leishmania donovani to macrophages. METHODS: The susceptibility of the macrophages treated with M1/70 and M18/2, toward Leishmania donovani promastigotes was investigated by determining the percentage of infected macrophages and the number of parasites in infected macrophages. RESULTS: The percentage of invasive macrophages and the number of invasive parasites were significantly decreased after the macrophages were treated with the McAbs. The invasion process of parasites into the macrophages was found decelerated as well. When macrophages were treated with both M1/70 and M18/2, the invasion of parasites into the macrophages was more distinctly inhibited than those treated with either of the two McAbs, the infection rate being 13.8% and only 1- 2 parasites could be found in each infected macrophage. CONCLUSION: The inhibitory effect of McAbs M1/70 and M18/2 on the invasion of Leishmania donovani to macrophages was achieved by blocking separately the specific binding sites of the macrophage surface molecules, Mac-1 receptor.

Key words: Leishmania donovani, macrophage, complement receptor