Abstract: Tbis paper recommends a simple and easy method to isolate and purify glyceraldehyde-3-phosphate dehydrogenase(GAPDH) from Schistosoma japonicum with electrophoretic puri-ty.The recovery rate of 5 6.1% of glyceraldehyde-3-phosphate dehydrogenase from S.japonicum extract was achieved by ammonium sulfate precipitation,DEAE-cellulose columnchromatography and SDS-PAGE.Using SDS-PAGE,the presence of one major band in thefinal purified GAPDH preparation was demonstrated.This protein had a MW of 37 kDa. The authers discuss the important functions of GAPDH and suggest that this method may beuseful in the research of the protective immunity of S.japonicum GAPDH in the developmentof schistosome vaccine.