CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2023, Vol. 41 ›› Issue (1): 117-120.doi: 10.12140/j.issn.1000-7423.2023.01.019

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Surveillance of resistance of Anopheles sinensis in some areas of Henan Province

HE Zhiquan1,2(), HU Yabo3, WANG Dan1,2, ZHANG Hongwei1,2,*(), LIU Ying1,2, YANG Chengyun1,2, QIAN Dan1,2, JI Penghui1,2, JIANG Tiantian1,2, LU Deling1,2   

  1. 1. Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China
    2. Henan Provincial Medical Key Laboratory of Parasitic Pathogen and Vector, Zhengzhou 450016, China
    3. College of Public Health, Zhengzhou University, Zhengzhou 450016, Henan, China
  • Received:2022-03-29 Revised:2022-07-01 Online:2023-02-28 Published:2023-02-24
  • Contact: * E-mail: zhwei69@163.com
  • Supported by:
    Henan Medical Science and Technology Plan Project(LHGJ20210145)

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Abstract:

To understand the resistance of Anopheles sinensis to insecticides, An. sinensis were captured near sheepfolds, pigstys or cowsheds in four counties (districts), including Pingqiao District of Xinyang City, Xiangfu District of Kaifeng City, Xiangcheng District of Xuchang City, and Tanghe County of Nanyang City in Henan Province from July to September 2021. The An. sinesis was identified by morphology. The insecticide susceptibility test of female An. sinensis was determined by the filter-paper bioassay, which is recommended by WHO, using 0.05% deltamethrin (0.017 8 g/m2), 0.15% beta-cyfluthrin (0.053 4 g/m2), 0.1% propoxur (0.035 7 g/m2), and 5% malathion (1.78 g/m2). The result showed that a total of 1 334 An. sinensis were used for insecticide susceptibility test in Pingqiao District, Xiangfu District, Xiangcheng County and Tanghe County. The mosquitoes were exposed to the four insecticide films, including, beta-cyfluthrin, deltamethrin, propoxur and malathion. The longest knockdown time of the first mosquito exposed to deltamethrin was seen in mosquitoes collected from Tanghe was 25.85 min, and the shortest knockdown time of the first mosquito exposed to deltamethrin was seen in mosquitoes collected from Pingqiao, which was 2.83 min. The knockdown rate of cypermethrin against the tested mosquitoes from Pingqiao District, Xiangfu District, Xiangcheng County and Tanghe County after 60 min was 24.42% (21/86), 2.47% (2/81), 37.31% (25/67) and 45.45% (25/55), respectively (χ2 = 39.355, P < 0.05）. The knockdown rate of deltamethrin against mosquitoes from all different sites were 75.86% (66/87), 2.33% (2/86), 10.81% (8/74) and 20.35% (23/113), respectively (χ2 = 142.630, P < 0.05). The knockdown rate of propoxur against mosquitoes from all different sites were 48.28% (42/87), 22.62% (19/84), 50.50% (51/101) and 72.34% (34/47), respectively (χ2 = 32.635, P < 0.05). The knockdown rate of malathion against mosquitoes from all different sites were 66.29% (59/89), 15.69% (16/102), 82.54% (104/126) and 91.84% (45/49), respectively (χ2 = 133.489, P < 0.05). The mortality rate of mosquitoes from Tanghe exposed to beta-cypermethrin and propoxur for 24 h was 70.91% and 80.60%, respectively, and the mortality rate of mosquitoes from Pingqiao exposed to deltamethrin for 24 h was 85.85%, which had reached resistance. The 24 h mortality rates of mosquitoes from Pingqiao and Tanghe to malathion were 97.43% and 97.67%. The resistance levels were possible resistance; The 24 h mortality rates of mosquitoes from Xiangfu and Xiangcheng to malathion were 64.23% and 99.21%. The resistance levels were resistance and susceptible. The resistance level of An. sinensis to malathion varies from susceptible to resistance but has developed resistance to deltamethrin, beta-cyfluthrin, and propoxur in four surveillance sites of Henan Province.

Key words: Anopheles sinensis, Insecticide, Resistance, Henan Province

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