Investigation on the insecticide resistance of Anopheles sinensis in Guizhou Province from 2018 to 2020

doi:10.12140/j.issn.1000-7423.2023.01.017

Abstract

Abstract:

To determine the insecticide resistance of Anopheles sinensis in Guizhou Province, A. sinensis was captured from the fields of Congjiang County, Bozhou District and Libo County of Guizhou in August 2018 to 2020. The mosquitoes were tested by the filter paper contact method, which is recommended by WHO. The susceptibility and insecticide resistance level of the captured mosquitoes to the diagnostic dose of 4% DDT, 0.05% deltamethrin and 5% malathion were evaluated. A Chi-square test was used to compare the knockdown rates after 60 min exposure and the corrected mortality rate between different study sites. After 60 min exposure, the knockdown time of the first mosquito to 0.05% deltamethrin was the longest in all study area, which takes 8.63 min, 17.52 min and 14.87 min in Congjiang, Bozhou and Libo; for 4% DDT, the knockdown time of the first mosquito was 6.33 min, 2.95 min and 11.88 min; for 5% malathion, the knockdown time of the first mosquito was 7.95 min, 2.15 min and 9.80 min, respectively. In Congjiang, Bozhou and Libo, after exposed for 60 min, the knockdown rates of tested mosquitoes to 5% malathion was the highest in all study areas, which were 83.9%, 39.0%, 59.0% respectively and there were significant differences in the knockdown rates among the three study areas (χ² = 53.98, P < 0.05). For 4% DDT, the knockdown rates were 61.9%, 33.3% and 36.2%; for 0.05% deltamethrin, the knockdown rates were 20.0%, 34.2%, 11.3%, respectively. The knockdown rates for 50% (KT₅₀) of tested mosquitoes in Congjiang, Bozhou, Libo for 4% DDT were 51.29 min, 127.65 min and 80.05 min; for 0.05% deltamethrin were 208.27 min, 74.69 min and 420.14 min; for 5% malathion were 29.33 min, 75.60 min and 42.19 min, respectively. The adjusted mortality rates of tested mosquitoes in Congjiang, Bozhou, Libo for 5% malathion were 90.1%, 95.0% and 95.9% respectively, which suggested the existence of resistance. There were no significant differences among the three study areas (χ² = 4.64，P > 0.05). For 4% DDT, the mortality rates were 65.6%, 38.6% and 55.0%; for 0.05% deltamethrin, the mortalityrates were 35.2%, 35.2% and 10.4%, respectively, which suggested that all tested mosquitoes had developed high level of resistance. This study suggested that An. sinensis in Guizhou Province have developed high level of resistance to deltamethrin, and maintained resistance to DDT and may have possible resistance to malathion.

Key words: Anopheles sinensis, Resistance, Insecticide, Guizhou

CLC Number:

R384.111

SHI Weifang, TIAN Zhenzao, ZHOU Jingzhu, HUANG Xueping, ZHOU Xuemei, WU Guoyan, LI Qiong, LIAO Qilang, WANG Dan. Investigation on the insecticide resistance of Anopheles sinensis in Guizhou Province from 2018 to 2020[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 108-111.

Figures/Tables 1

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