关键词: 恶性疟原虫, 重组蛋白, 复合抗原, 体外抑制试验, 免疫血清

Abstract: The inhibitory effects of rabbit immune sera against two recombinant hybrid antigens(C and CAC) of Plasmodium falciparam to the multiplication and development of in vitro cultured P.falciparum parasites were tested. Although both of anit-C and anti-CAC immune sera had inhibitory effect on parasites, the anti-CAC serum showed much greater effect than that of anti-C serum(P0. 05). The inhibitory capacities in the sera to parasites were enhanced with the increase in the serum concentration as well as with the prolongation of the incubation time of the sera with the parasites. It was shown that after 72 h incubation of the anti-CAC serum with the parasites at the concentration of 1 %, 10% and 20%, the inhibition rates were 15%, 54% and 82%, respectively. The immune sera caused the dispersion of parasite cytoplasm, atrophy of parasites, agglutination of free merozoites and degeneration of schizonts, suggesting that the hybrid antigen could produce multi-functional protective antibodies against antigens of Plesmodium falciparum.