Table of Content

30 December 1999, Volume 17 Issue 6

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论著

INDUCTION OF PROTECTIVE IMMUNITY IN MICE AGAINST SCHISTOSOMA JAPONICUM BY NUCLEIC ACID VACCINE ENCODING THE FULL-LENGTH PARAMYOSIN*

ZHOUShenghua;LIUShuxian;SONGGuangcheng;XUYuxin;SUNWenyu

1999, 17(6):  1-324. 

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　AIM: To investigate the immune efficacy of nucleic acid vaccination in mice against full-length paramyosin of Chinese Schistosoma japonicum. METHODS: C\-\{57\}BL/6 and BALB/c mice were vaccinated intramuscularly with the nucleic acid vaccine (pCMV-SjC97) encoding the full-length gene of paramyosin of Chinese S.japonicum. Each group was immunized three times at weeks 0, 3 and 6. Mice vaccinated with pCMV blank vector served as negative control. Mice were challenged three weeks after final DNA boosting by percutaneous infection with cercariae. Six weeks after infection the mice were perfused, worm burden and eggs in the livers, spleens and intestines were counted. Sera from vaccinated mice were collected from the tail vein at weeks 0, 3, 6 and 9, respectively. RESULTS: C\-\{57\}BL/6 mice vaccinated with pCMV-SjC97 produced predominantly IgG2a and IgG2b; whereas in BALB/c mice, IgG1, IgG2a and IgG2b antibodies. Immunization with the pCMV-SjC97 in C\-\{57\}BL/6 mice could confer significant worm reduction rate (35.5%-41.4%,P<0.05) and egg reduction rate (liver:44.5%-59.6%, P<0.05; spleen:56.7%-82.4%, P<0.05; intestines:57.9%, P<0.05), but not in BALB/c mice. CONCLUSION: The nucleic acid vaccine, pCMV-SjC97, could induce protective immunity in C\-\{57\}BL/6 mice significantly. \;


STUDIES ON IMMUNE RESPONSES IN MICE TO EXPRESSED PRODUCTS FROM CSP GENE OF PLASMODIUM FALCIPARUM FCC1/HN ISOLATE\+*

FANGZheng**;YUXinbing;LIUYanwen;LUOShuhong

1999, 17(6):  2-329. 

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　AIM: To construct the eukaryotic expression system with pcDNA3-PfCSP/HeLa for CSP gene of \{Plasmodium\} falciparum, and to observe the immune responses in BALB/c mice induced by the expressed proteins. \{METHODS\}: The recombinant plasmid pcDNA3-PfCSP was transformed into mammalian cell line of human HeLa cells. The expressed protein was isolated and analyzed by SDS-PAGE and used for immunization of BALB/c mice by subcutaneous, intravenous or intraperitoneal administration, respectively. ELISA, Western blotting, T lymphocyte proliferation test, natural killer cell activity and CD4\++ and CD8\++ T cells detection were used for observation of the level of humoral and cellular immune responses. RESULTS: Immune sera strongly reacted with the expressed protein. The titer of the antibodies was up to 1∶6 400 by ELISA. Western blotting analysis revealed a specific band at 38.3 kDa. The specific proliferative response with immunized BALB/c mice spleen cells was remarkablely higher than that with control ones. The ratio of CD4\++ and CD8\++ T cells and the NK cell activity were significantly higher in the immunization group than that in the control groups. CONCLUSION: The humoral and cell-mediated immune responses and elevated NKC activity to expressed products with the eukaryotic expression system can specifically be detected in BALB/c mice to generate, indicating that the expressed protein could enhance immune activity in mice. \;


EFFECTS OF IFN-γ GENE-MODIFIED HEPATOCYTES ON TGF-β_1 AND ITS RECEPTOR IN MICE INFECTED WITH SCHISTOSOMA JAPONICUM*

ZHANGLihuang;YAOHangping;CAOXuetao;YUYizhi;CHENHong;LIMinwei

1999, 17(6):  3-333. 

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　AIM: To explore the anti-schistosomal hepatic fibrosis effect and the changes in transforming growth factor-β\-1 (TGF-β\-1) and its receptors (TGF-βRII) in S.japonicum infected mice after intrasplenic transplantation of γ-interferon (IFN-γ) gene-modified hepatocytes. METHODS: At 16 wk after infection with cercariae of Schistosoma japonicum, the mice were intrasplenically transplantated with murine hepatocytes which had been transfected with IFN-γ gene-combinant adenovirus vector. ELISA, immunohistochemical and dot blot techniques were used to observe the dynamic changes in IFN-γ, TGF-β\-1, TGF-βRII and typeⅠ,Ⅲ collagen. RESULTS: The intrasplenic transplantation of IFN-γ gene modified hepatocytes effectively expressed IFN-γ and obviously reduced the production and deposition of typeⅠ,Ⅲ collagen as well as TGF-β\-1 and TGF-βRII. CONCLUSION: \{IFN-γ\} gene transplantation has anti-hepatic fibrosis efficacy in Schistosoma japonicum- infected mice, being related to its role of decreasing the expression of TGF-β\-1 and TGF-βRII.\;


IMMUNE RESPONSES IN MICE VACCINATED WITH RECOMBINANT PLASMID pcDNA3 CONTAINING ROP1 GENE FROM TOXOPLASMA GONDII*

GUOHong;CHENGuanjin;ZHENGHuanqin;ZHOUYongan;LUFangli

1999, 17(6):  4-337. 

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　AIM: To observe the immune responses in BALB/c mice vaccinated with the constructed recombinant plasmid, pcDNA3, containing ROP1 gene from Toxoplasma gondii. METHODS: The plasmid DNA obtained by alkaline lysis were injected into the left leg of each mouse at a dosage of 100 μg. A booster vaccine was given using the same dosage two weeks later. Control groups were injected with the pcDNA3 blank plasmid and normal saline, respectively. 30, 50 and 70 days after the booster injection, the proliferation activity of T lymphocytes and the NK cell activity were determined using MTT assay, the number of CD4+/CD8+ T cells with indirect immunofluorescence assay and the titer of IgG antibody by ELISA. RESULTS: The spleens of mice enlarged obviously after immunization with pcDNA3-ROP1. The activity of proliferation of spleen T lymphocytes was higher in the immunization group than in the control groups; The NK cell activity in the immunization group was higher than those of control ones. The number of CD4+ T cells showed no obvious increase but CD8+ T cells obviously increased. The titer of IgG antibody in the immunization group showed no significant increase until 90 days later. CONCLUSION: Vaccination of mice with the recombinant plasmid, pcDNA3-ROP1, could elicit cellular and humoral immune response in mice. \;


ESTABLISHMENT AND IDENTIFICATION OF THE MONOEPITOPIC MONOCLONAL ANTIBODY OF PLASMODIUM FALCIPARUM*

QUJingqi;YANGBolin;BAOYifang;YANGYuetao;XUYongxiang;XUXuenian;FANPeichang;RENYiping

1999, 17(6):  5-341. 

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　AIM: To prepare a monoepitopic monclonal antibody of Plasmoduim falciparum. METHODS: According to the theory of protein structure, a peptide with 6-9 residues representting the antigenicity of the original histidine-rich protein II (HRP-II) of P.falciparum was synthesized and used to immunize BALB/c mice after purity identification by capillary electrophoresis. RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtaind by using spleen embedment method and hybridoma technology. CONCLUSION: It is the first report to prepare a monoepitopic monoclonal antibody against original protein by selecting a suitable peptide from the primary sequence of protein.\;


EFFECT OF TRICLABENDAZOLE ON THE ULTRASTRUCTURE OF BODY WALL AND VITELLINE CELLS OF PARAGONIMUS WESTERMANI*

XUShi′e;CHENCaiyun;JINLiqun;LUXiujun;CHENJiajun

1999, 17(6):  6-345. 

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　AIM: To observe the ultrastructural changes in the body wall and the vitelline cells of Paragonimus westermani in vitro and in vivo before and after triclabendazole treatment. METHODS: The worms were obtained from in vitro and in vivo tests. All of the samples were processed by conventional techniques, and observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). RESULTS: The external plasma membrane and matrix were cracked or disappeared after the treatment. The necrosis of the muscular layer differed. The cell membranes of cortex and vitelline cells were damaged. Nuclear membrane was damaged partially, heterochromatin solidified and condensed to brim and dissolved. The Golgi complex disappeared, endoplasmic reticulum expanded, mitochondria denatured and dissolved. The damage was more serious in vivo than in vitro. CONCLUSION: Triclabendazole is remarkablely effective against Paragonimus westermani by damaging the body wall and vitelline cells, mainly affecting the nuclei, membrane structures and microtubular system.\;


ANALYSIS OF KINETOPLAST DNA OF LEISHMANIA ISOLATES IN CHINA BY PCR-SSCP

ZHENGXueli;\HUXiaosu;CHENJianping

1999, 17(6):  7-349. 

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　AIM: To analyse the kDNA of the pathogens of leishmaniasis in China. METHODS: Based on leishmaniasis specific primers 13A, 13B and a set of oligonucleotide primers Ⅰ and Ⅱ with Leishmania donovani (L.d.) Sichuan isolate specificity, PCR were conducted to amplity minicircle kDNA fragments (297 bp and 120 bp) in the pathogens of leishmaniasis from different epidemiologic foci in China. The products were analyzed by single strand conformation polymorphism technology (SSCP). RESULTS: PCR amplified 297 bp product occurred in L.d. isolates from hill and desert foci, but no product was found in L.d. isolates from plain foci in China. SSCP of these 297 bp kDNA fragments showed that there was no difference in the mobility of ssDNA between isolates from hill foci, but there was apparent difference in the mobility of ssDNA between L.d. isolates from hill and desert foci. PCR amplified 120 bp products occurred in L.d. Sichuan isolate, L.d. Wenchuan isolate, L.d. Gansu isolate from hill foci and L.d. Shandong isolate and L.d. Jiangsu isolate from plain foci. SSCP of the 120 bp products showed that no difference in the mobility of ssDNA was found between two isolates from plain foci. There was also no difference in the mobility of ssDNA between L.d. Wenchuan isolate and L.d. Gansu isolates from hill foci. But there was apparent difference in the mobility of ssDNA between L.infantum and L.d. isolates from different foci. CONCLUSION: Heterogeneity does exist between the kDNA of L.d. isolates from different foci of leishmaniasis of China.\;


ANTI-TOXOPLASMA EFFECT OF ACTIVATED MOUSE MACROPHAGES INDUCED BY INTERFERON-γ COMBINED WITH TNF-α*

ZHANGAimin;YANGHuizhen;YANGYang;QIANZhongli

1999, 17(6):  8-352. 

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　AIM: To compare the inhibitory effect of the macrophages activated by IFN-γ combined with TNF-α against RH strain and Fukaya strain. METHODS: The average parasite proliferation rates of the two strains within the cytokine-activated MΦs were calculated at different times post-challenge, the nitric oxide (NO) levels in the medium supernatant were simultaneously determined. RESULTS: In the macrophages activated by 100 U each of IFN-γ and TNF-α, the invaded tachyzoites of RH strain were completely killed, while the invaded tachyzoites of Fukaya strain remained slow proliferation with significantly lower levels of NO detected at 24 h post challenge. CONCLUSION: The difference in the anti-Toxoplasma effect of the activated macrophages against RH and Fukaya strains might be attributed to the different amount of NO produced by the macrophages.\;


COMPARISON OF SENSITIVITY OF ARTESUNATE-SENSITIVE AND ARTESUNATE-RESISTANT PLASMODIUM FALCIPARUM TO CHLOROQUINE AND AMODIAQUINE*

YANGHenglin;GAOBaihe;HUANGKaiguo

1999, 17(6):  9-355. 

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　AIM: To explore the in vitro sensitivity of artesunate-sensitive and -resistant Plasmodium falciparum to chloroquine and amodiaquine and to observe the effect of artesunate combined with chloroquine and artesunate combined with amodiaquine on artesunate-resistant P.falciparum. METHODS: The sensitivity of the artesunate-sensitive and -resistant P.falciparum to chloroquine and amodiaquine and their combination with artesunate was compared by using Rieckmann′s in vitro micro-technique.RESULTS: The ID\-\{50\} and ID\-\{95\} values of chloroquine, amodiaquine and artesunete were 90.9, 50.9, 9.6 nmol/L and 320.0、320.0, 40.0 nmol/L to the artesunate-sensitive P.falciparum respectively and were 112.0, 133.5, 85.1 nmol/L and 320.0, 320.0, 400.0 nmol/L to artesunate-resistant P.falciparum, respectively. When artesunate was combined with chloroquine, the ID\-\{50\} values of the 2 drugs were 3.2 and 20.0 nmol/L to the artesunate-resistant P.falciparum. When artesunate was combined with amodiaquine, the ID\-\{95\} values of the 2 drugs were 3.2 and 5.0 nmol/L to the artesunate-resistant P.falciparum, respectively. CONCLUSION: The artesunate-resistant P.falciparum has no cross resistance to chloroquine and amodiaquine, however, artesunate combined with chloroquine or amodiaquine exhibit an apparent synergistic effect in vitro.\;


DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN

HEJianwen;CAIXianzheng;ZHANGYongsheng;WANGXiangfeng;HUADe;WANGSaimei

1999, 17(6):  10-358. 

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　AIM: To establish a modified polymerase chain reaction(PCR) method for the detection of Plasmodium vivax in the endemic areas of malaria and compare the method with the conventional light microscopy in the field.METHODS: A PCR method was modified by improving the collection procedures of blood samples, template extraction, primer design and optimizing the reaction condition. The method was evaluated by examining blood samples from 310 patients with vivax malaria and compared with the conventional light microscopy in endemic areas of Hainan Province. RESULTS: The positive rates of the modified PCR method and microscopic method were 34.2% and 31.9%, respectively. CONCLUSION: The modified PCR method is simple, sensitive and specific for the detection of vivax malaria patients in endemic areas. \;


EXPRESSION OF PLASMODIUM FALCIPARUM-INFECTED ERYTHROCYTE MEMBRANE PROTEIN FROM CEREBRAL MALARIA PATIENTS

BIANZhongqi;WANGGongzhuo;TIANXueming;FANJiang

1999, 17(6):  11-362. 

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　AIM: To provide theoretical cvidence for studying the molecular pathogenesis of human cerebral malaria.METHODS: The expressions of Plasmodium falciparum erythrocyte membrane protein 1(PfEMP1) on the surface of parasitized erythrocyte (PE) specimens from 19 cases of cerebral malaria patients in Yunnan Province were quantitatively analyzed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique. 43 patients of falciparum malaria, 9 patients of vivax malaria and 6 healthy controls were also investigated.RESULTS: The expressions of higher molecular mass (Mr) 260-320 kDa forms of PfEMP1 were found on PE from cerebral malaria patients. By contrast, the expression of PfEMP1 and P.vivax erythrocyte membrane protein (PvEMP1) on PE from falciparum malaria patients and vivax malaria patients had a PfEMP1 with Mr 240 kDa and a PvEMP1 with Mr 180 kDa band, respectively. Healthy controls expressed an EMP of Mr 140 kDa. CONCLUSION: The binding of 260-320 kDa PfEMP1 proteins expressed on PE from cerebral malaria patients to diverse receptor molecules on the endothelial cell(EC)of the cerebral microvessels such as CD36, thrombospondin (TSP), intercellular adhesion molecule 1(ICAM-1), vascular cell adhesion molecule 1(VCAM-1), endothelial leukocyte adhesion molecule 1(ELAM-1) and chondroitin sulfate A (CSA) might be the molecular basis for the pathogenesis of cerebral malaria.\;


FINDING OF NEW FCC1/HN ANTIGENIC EXPRESSED SEQUENCE TAG(ESTs) OF PLASMODIUM FALCIPARUM

YANZhonghe;XIEYi;LIMing;WANGPing;WANGYanni;BIHuixiang;LIYingjie

1999, 17(6):  12-366. 

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　AIM: To sequence the strong positive clones obtained by immuno-screening of Plasmodium falciparum FCC1/HN λgt11 cDNA expression library, and to elucidate the antigenic expressed sequence tags through sequencing the cDNA insert of these positive clones, and new antigenic ESTs could serve as a resource to pursue their corresponding antigen genes. METHODS: cDNA inserts of positive λgt11 phage clones were amplified by PCR. The PCR products, after purification, were cloned into the M13 mp18 sequencing vector. Single-stranded M13 DNA was prepared and sequenced. Then the acquired sequences were compared in homologies with EMBL/GenBank database on the PC/GENE software system and searched in NCBI (National Center for Biotechnology Information) GenBank using BLAST (Basic Local Alignment Search Tool) commond. RESULTS: Sequence C03 was part of the known P.falciparum antigenic heat shock protein 70 (Pfhsp70) gene, while the other 5 sequences were new P.falciparum antigenic expressed sequence tags (ESTs). CONCLUSION: The 5 new antigenic ESTs generated could serve as the breaking through points in our efforts to find out new P.falciparum antigen genes.


SDS-PAGE AND EITB ANALYSIS OF THE PROTEIN COMPONENTS OF DIFFERENT ISOLATES OF SCHISTOSOMA JAPONICUM IN CHINA AND JAPAN *

QIULishu;LIUSen;XUEHaichou;ZHANGYonghong;LIHao;HUYaqing;HEYixun

1999, 17(6):  13-369. 

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　AIM: To make a supplementary observation on the protein components prepared from adult S.japonicum of 4 different isolates from Zhejiang, Jiangxi, Taiwan Provinces of China and Japan in origin and to observe antigen reactivity of the above-mentioned isolates together with adult worms from Anhwi, Hubei, Sichuan and Yunnan isolates against heterologous anti- Oncomelania h.hupensis (collected from Guangxi, China and Japan) sera by EITB. METHODS: SDS-PAGE and EITB.RESULTS AND CONCLUSION: SDS-PAGE showed that by Coomassie blue staining,male S.japonicum from Jiangxi, Zhejiang, Taiwan Provinces and Japan isolales revealed 7-17 bands while female worms revealed 1-6 bands. The protein patterns of Taiwan and Zhejiang male worm were similar, but slight difference could be seen above 81 kDa. By silver staining, male worms of the 4 isolates revealed 10-23 bands while female worms revealed 1-19 bands. Male worms from Japan not only showed less bands but also differed in their pattern as compared to those of other Chinese isolates. Results of EITB revealed that each of the 4 isolates had slightly different pattern but all of the tested isolates had common antigens with their heterologous snail hosts i.e., Oncomelania h.hupensis from Guangxi, China and Japan.


BIOLOGICAL CHARACTERISTICS OF THE JUVENILE PARAGONIMUS WESTERMANI

YANTao;LIGuoliang

1999, 17(6):  14-373. 

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　AIM: To observe the biological characteristics of the juvenile of Paragonimus westermani. METHODS: Kunmin strain mice and Wistar rats inoculated orally with 100-150 metacercariae of Paragonimus westermani were autopsied 10-30 days and 80-580 days after infection, respectively. Life-span, survival time in dead hosts or physiological saline at 5℃ to 8 ℃ and invasiveness of juveniles to new hosts were detected by host transfer. RESULTS: All the worms recovered from the mice and rats were stunted juveniles. The worm detection rates in the mice and rats were 19.3% and 22.2%-37.5%, respectively. The life-span of the juveniles was rather long, being not limited by the life-span of a host. Host transfer could prolong longevity and preserve invasiveness. The juveniles recovered from dead mice still possess strong vitality and invasiveness. CONCLUSION: The juvenile of P.westermani is an infective stage, having strong vitality and invasiveness.\;


实验研究

IMMUNOREGULATION OF IL-2 IN TRICHINELLA-INFECTED MICE

NIUChun;JIANGHongjie;HUANGSong;ZHUXinping

1999, 17(6):  15-376. 

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　AIM: To study the immunoregulation of IL-2 in Trichinella-infected mice. METHODS: Mice infected respectively with HL strain and AM strain larvae of Trichinella spiralis were treated ip with IL-2 from the second day postinfection for 3 days. Serum IgG antibody levels were determined by ELISA and the infection capacity was determined using reproductive capacity index (RCI). RESULTS: In HL, higher dosage of IL-2 injection induced lower RCI and showed apparent anti-Trichinella effect. In AM, both low and high dose of IL-2 had no measurable effect on RCI, however, high dose of IL-2 reduced the infectivity of newborn larvae. CONCLUSION: IL-2 exhibits apparent suppressive effect on the infectivity of T.spiralis of HL strain.\;


DYNAMIC CHANGES IN IL-4, IL-5 AND IL-10 IN LIVER AND BONE MARROW OF MICE INFECTED WITH SCHISTOSOMA JAPONICUM*

ZENGLinglan;LUODuande;LIUWei;GUOJingsong;LIShuli

1999, 17(6):  16-379. 

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　AIM: To observe the changes in Th2 cytokines in the liver and bone marrow of mice infected with schistosomiasis japonica. METHODS: ABC immunohistochemical staining technique and multimedia pathological picture analysis were used. Meanwhile, IL-4, IL-5 and IL-10 derived from livers and bone marrows of infected mice were observed at wk 8, 10 and 12 after infection. RESULTS AND CONCLUSION: In the livers of infected mice, the levels of IL-4, IL-5 and IL-10 increased obviously with a prolongation of the infection duration, IL-4 being the highest. However, in the bone marrow of infected mice, IL-4 level increased slowly with the duration of the infection, being lower than those in the liver at wk 10 and 12 after infection. The level of IL-5 was higher than that in the liver within 12 wk. At wk 10 after infection IL-10 tended to increase, but decreased thereafter and was obviously lower than that in the liver. In mice infected with Schistosoma japonicum, the immune response occurred mainly in the liver. IL-5 in the bone marrow showed auto-secretion. \;


STUDIES ON DETECTION OF PLASMODIUM FALCIPARUM AND PLASMODIUM VIVAX IN BLOOD SAMPLES BY MULTIPLEX POLYMERASE CHAIN REACTION

CHENShu;LUHuimin;GAOQi;TANGXueheng

1999, 17(6):  17-383. 

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　AIM: To establish a sensitive and specific PCR-based method to detect Plasmodium falciparum and P.vivax in blood samples in a single amplification reaction. METHODS: Malaria parasite DNA in blood was amplified by the multiplex polymerase chain reaction using two sets of primers derived from the P.f. moderately-repetitive DNA sequence and COIII gene of P.v. RESULTS: A 206- bp product for P.f. and a 370- bp product for P.v. were amplified by multiplex PCR, being able to detect parasitemia level as low as 5×10\{-7\} for P.f. and 1.02×10\{-6\} for P.v. and having no cross-reaction with human leucocyte DNA. A total of 783 blood samples on the filter paper collected from patients attending to malaria clinics in malaria endemic areas were detected. The positive rate of multiplex PCR was 85.8%, the misdiagnosis rate was 0, and the under-diagnosis rate was 0.1%, while these three rates of microscopic examination were 84.9%, 3.1% and 1.0%, respectively. The concordance between the two methods was 95.8%. CONCLUSION: The multiplex PCR method made the malaria detection more sensitive and specific than the microscopic examination and should be suitable for the diagnosis of malaria in mixed endemic areas, large-scale epidemiological studies, follow-up of drug treatment and donor blood screenig.\;


CELLULAR IMMUNE RESPONSE IN MICE VACCINATED WITH UV-ATTENUATED TOXOPLASMA GONDII ZS1 STRAIN TROPHOZOITES*

LUFangli;ZHENGHuanqin;GUOHong;CHENGuanjin

1999, 17(6):  18-386. 

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　AIM: To explore the protective effect and cellular immune response of uv-attenuated ZS1 strain trophozoites of Toxoplasma gondii in mice. METHODS: The ZS1 strain trophozoites of T.gondii were irradiated by uv-light with 2537 \{°A\} wave length for 60 minutes. Mice were divided into 4 groups. Group 1 was vaccinated alone, group 2 was challenged with normal ZS1 trophozoites on d45 after vaccination, group 3 was infected alone, and group 4 was normal control. The changes in splenic T cell proliferation, level of CD4+ and CD8+ T cell, and NK cell activity were compared. RESULTS: Group1 mice survived normally, no trophozoite,pseudo-cyst or cyst was detected in the tissues on d49 after vaccination. Group 2 mice survived longer than those of group 3. The T lymphocyte proliferation in response to soluble antigen of T.gondii was significantly enhanced in group 2, and suppressed in group 3. The level of CD4+ T cell in group 2 was decreased, resulting in a reverse of CD4+/CD8+ ratio. The NK cell activities in groups 1, 2 and 3 were all significantly increased. CONCLUSION: The uv-attenuated vaccine of T.gondii ZS1 strain could induce certain protective immunity against challenge infection, in which CD8+ T cell and NK cell might play an important role.\;


THE PROTECTIVE EFFECT AGAINST TOXOPLASMA INFECTION IN MICE IMMUNIZED WITH LASER-IRRADIATED TOXOPLASMA TACHYZOITES*

LINAifen;LUShaohong;CHENCaihua;LISiwen;CHENRui;LUXuanhui;HUANGFuquan

1999, 17(6):  19-389. 

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　AIM: To observe the level of immune responses and protective immunity in mice induced by laser-irradiated Toxoplasma tachyzoites (LIT).METHODS: ICR mice were immunized with LIT. After one month, the mice were challenged with live Toxoplasma tachyzoites to observe the effect of LIT. Indirect immunofluorescence assay and ELISA were used to detect CD4+/CD8+ lymphocyte subpopulation and IgG antibody. RESULTS: Immunization with LIT could partially protect the mice from Toxoplasma infection, prolong the survival time, enhance the CD4+/CD8+ ratio and produce specific IgG antibody. CONCLUSION: Intraperitoneal injection with LIT can induce partial protective immunity and specifically elicit ICR mice to generate humoral and cell-mediated immune responses.\;


临床研究

CHANGES IN COMPUTED TOMOGRAM IN CEREBRAL PARENCHYMAL CYSTICERCOSIS TREATED WITH ALBENDAZOLE

ZHAOShousong;XUKuihua

1999, 17(6):  20-393. 

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　AIM: To observe the changes in cerebral computed tomogram (CT) in cerebral parenchymal cysticercosis after albendazole treatment. METHODS: Cerebral CT scanning was conducted in 57 patients with cerebral cysticercosis in our hospital before, during and after albendazole treatment. RESULTS: Cerebral CT might be normal before onchospheres became cysticercariae in the brain. Small cystic lesions could turn into other CT signs of cerebral cysticercosis after albendazole treatment. CONCLUSION: Normal cerebral CT cannot rule out the disease. Small cystic lesions are the earliest pathological and active signs of the disease. Nodular focus occurs after the death of Cysticercus and calcification is the final outcome of the disease. \;