Abstract: 　AIM: To prepare a monoepitopic monclonal antibody of Plasmoduim falciparum. METHODS: According to the theory of protein structure, a peptide with 6-9 residues representting the antigenicity of the original histidine-rich protein II (HRP-II) of P.falciparum was synthesized and used to immunize BALB/c mice after purity identification by capillary electrophoresis. RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtaind by using spleen embedment method and hybridoma technology. CONCLUSION: It is the first report to prepare a monoepitopic monoclonal antibody against original protein by selecting a suitable peptide from the primary sequence of protein.\;

Key words: Histidine-rich protein II, synthetic peptide, monoepitopic monoclonal antibody