Abstract: 　AIM: To explore the anti-schistosomal hepatic fibrosis effect and the changes in transforming growth factor-β\-1 (TGF-β\-1) and its receptors (TGF-βRII) in S.japonicum infected mice after intrasplenic transplantation of γ-interferon (IFN-γ) gene-modified hepatocytes. METHODS: At 16 wk after infection with cercariae of Schistosoma japonicum, the mice were intrasplenically transplantated with murine hepatocytes which had been transfected with IFN-γ gene-combinant adenovirus vector. ELISA, immunohistochemical and dot blot techniques were used to observe the dynamic changes in IFN-γ, TGF-β\-1, TGF-βRII and typeⅠ,Ⅲ collagen. RESULTS: The intrasplenic transplantation of IFN-γ gene modified hepatocytes effectively expressed IFN-γ and obviously reduced the production and deposition of typeⅠ,Ⅲ collagen as well as TGF-β\-1 and TGF-βRII. CONCLUSION: \{IFN-γ\} gene transplantation has anti-hepatic fibrosis efficacy in Schistosoma japonicum- infected mice, being related to its role of decreasing the expression of TGF-β\-1 and TGF-βRII.\;

Key words: interferon, gene therapy, schistosomiasis, transforming growth factor-β\-1, transforming growth factor receptorII