›› 1999, Vol. 17 ›› Issue (6): 8-352.

• 论著 • Previous Articles     Next Articles

ANTI-TOXOPLASMA EFFECT OF ACTIVATED MOUSE MACROPHAGES INDUCED BY INTERFERON-γ COMBINED WITH TNF-α*

ZHANG Aimin1;YANG Huizhen2;YANG Yang2;QIAN Zhongli2


  

  1. 1 Department of Parasitology;Medical School;Shanghai Tiedao University;Shanghai 200331 2 Department of Parasitology;Shanghai Second Medical University;Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-12-30 Published:1999-12-30

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Abstract: 　AIM: To compare the inhibitory effect of the macrophages activated by IFN-γ combined with TNF-α against RH strain and Fukaya strain. METHODS: The average parasite proliferation rates of the two strains within the cytokine-activated MΦs were calculated at different times post-challenge, the nitric oxide (NO) levels in the medium supernatant were simultaneously determined. RESULTS: In the macrophages activated by 100 U each of IFN-γ and TNF-α, the invaded tachyzoites of RH strain were completely killed, while the invaded tachyzoites of Fukaya strain remained slow proliferation with significantly lower levels of NO detected at 24 h post challenge. CONCLUSION: The difference in the anti-Toxoplasma effect of the activated macrophages against RH and Fukaya strains might be attributed to the different amount of NO produced by the macrophages.\;

Key words: Interferon-γ, tumor necrosis factor-α, Toxoplasma gondii, marcophage, nitric oxide