DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN

Abstract

Abstract: 　AIM: To establish a modified polymerase chain reaction(PCR) method for the detection of Plasmodium vivax in the endemic areas of malaria and compare the method with the conventional light microscopy in the field.METHODS: A PCR method was modified by improving the collection procedures of blood samples, template extraction, primer design and optimizing the reaction condition. The method was evaluated by examining blood samples from 310 patients with vivax malaria and compared with the conventional light microscopy in endemic areas of Hainan Province. RESULTS: The positive rates of the modified PCR method and microscopic method were 34.2% and 31.9%, respectively. CONCLUSION: The modified PCR method is simple, sensitive and specific for the detection of vivax malaria patients in endemic areas. \;

Key words: Plasmodium vivax, PCR, detection

HEJianwen;CAIXianzheng;ZHANGYongsheng;WANGXiangfeng;HUADe;WANGSaimei. DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN[J]. , 1999, 17(6): 10-358.

[1]	TANG Qi, LV Chao, WANG Xi, GUO Suying, XU Xiaojuan, ZHU Hai, LI Yinlong, LIN Weina, ZHOU Xinjie, FENG Ting, XU Jing, QIN Zhiqiang. Efficiency evaluation of three assays for detection of Schistosoma japonicum infections in wild mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 186-191.
[2]	YANG Wanxuan, SHEN Haimo, CHEN Shenbo, CHEN Junhu. Analysis of the key sites of Plasmodium vivax VIR14 protein interacting with ICAM-1 receptor [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 192-197.
[3]	WANG Ying, LIU Baixue, WANG Xu, WANG Liying, XUE Chuizhao, KUI Yan, YANG Shijie, WU Weiping, HAN Shuai. Advances in establishment of a national echinococcosis reference laboratory network in China [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 119-123.
[4]	SONG Shaozheng, MENG Yaqin, WU Yingchao, YU Kangying, ZHOU Zhe. Establishment and application of a recombinant polymerase amplification method for detecting Otodectes cynotis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(6): 802-805.
[5]	XU Guolei, FENG Yanye, HU Wei. Establishment and application evaluation of a rapid visualization detection method for Schistosoma japonicum specific nucleic acid fragments based on RPA-CRISPR/Cas12a technology [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(5): 608-614.
[6]	WANG Zhenyu, JIANG Li, YU Qing, ZHANG Yaoguang, WU Huanyu, CHEN Jian, ZHU Min. Interpretation of the Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 521-524.
[7]	LI Jing, YANG Shufeng, GAO Wenwei, LIU Qing, ZHU Xingquan, ZHENG Wenbin. Infection status and genotype identification of Toxoplasma gondii in pigs in Jinzhong City, Shanxi Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 367-371.
[8]	YI Jia, WU Dongni, DONG Xiaorong, ZHU Hong, LIN Wen, ZHANG Cong, SUN Lingcong. Analysis of laboratory detection capability for imported malaria in Hubei Province before and after malaria elimination [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 177-181.
[9]	ZHOU Shuimao, JIA Xishuai, YANG Yan, ZHANG Juan. Establishment of loop-mediated isothermal amplification detection for Paragonimus westermani [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 121-124.
[10]	MA Zhiya, XIE Shichen, HE Yuanhui, GAO Wenwei, LIU Qing, ZHU Xingquan, ZHENG Wenbin. Infection status and genetic variation analysis of Haemonchus contortus in sheep in Shanxi Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 733-738.
[11]	YUAN Huizhen, LI Dongliang, CHENG Shuqi, JIAN Fuchun. Study on the characteristics of Blastocystis cultured in vitro [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 631-635.
[12]	DING Hongyun, DONG Ying, XU Yanchun, DENG Yan, LIU Yan, WU Jing, CHEN Mengni, ZHANG Canglin. Polymorphism analysis of multidrug resistance protein 1 gene in imported Plasmodium vivax in Yunnan Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 404-411.
[13]	XU Yin, LIU Ting, XU Hui, ZENG Xiaojun, LAN Weiming, GONG Zhihong, DAI Kunjiao, QIU Tingting, HAO Xian, XIE Shuying. Establishment and application of PCR-CRISPR/Cas12a-based detection method for Clonorchis sinensis metacercaria [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 421-426.
[14]	CAI Zhanghuang, ZHANG Zhiping, ZHUO Mingying, GUO Liping, FU Zhihui, LIU Yiruo. Trematode egg internal transcribed space-2 sequence based analysis for diagnosis of Opisthorchis viverrin and Opisthorchis sinensis infections [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 427-433.
[15]	LI Yuqiong, YU Youli, GAO Junrong, LIU Yunyun, LI Hongbing, NIU Xiaohao. Enterocytozoon bieneusi infection in dairy cows and its genotype identification in Yinchuan area of Ningxia Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 476-479.

DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments