Table of Content

30 October 1999, Volume 17 Issue 5

Previous Issue    Next Issue

论著

SAFETY ANALYSIS OF DUST MITE ALLERGEN FOR DIAGNOSIS AND IMMUNOTHERAPY OF ASTHMA AND RHINITIS 

WENTinghuan;CAIYingyun;CHENXiujuan;XIANGLi;WANGBeiling;ZHUANGYijun

1999, 17(5):  6-276. 

Asbtract ( )   PDF (192KB) ( )  

Related Articles | Metrics

　AIM: To make a retrieval investigation of safety in using Dermatophagoides farinae extract in diagnosis and immunotherapy with patients of asthma and rhinitis.METHODS: A questionnaire evaluation of the safety use of D farinae extract ( SMU Df) during diagnosis and immunotherapy of patients from 1974 to 1995 was carried out. RESULTS: A total of 846 342 injections were statistically analysed,among whom 142 systemic adverse reactions occurred involving urticaria 0.82, severe attack of asthma 0 77, anaphylactic shock 0 07 (CL=1.4～12 0/million), and angioedema 0 02. The time of onset of systemic reactions of immediate type was <30 min in 32 cases times, and 1 h and 2 h in 6 cases times; the time to onset of late response type was 3 h～48 h in 23 cases times with highest incidence of severe asthmatic attacks. The systemic reactions occurred in 18 subjects during skin test, in 96 cases times during increasing dose phase, and in 14 cases times during. Among them 6 cases were of anaphylactic shock, and none of it after emergency treatment. The major cause of manifestation of systemic reactions for 41 cases after immunotherapy with SMU Df extract was due to over dosage errors. CONCLUSION: The application of SMU Df extract in skin test and immunotherapy of asthma and rhinitis sensitive to mite for the past 22 years in this country indicated that the efficacy and safety have been high.


CLONING AND SEQUENCING OF THE GENES CODING FOR THE HISTIDINE RICH PROTEIN II OF PLASMODIUM FALCIPARUM *

FANGJianmin;YUXinbing;LUOShuhong;XUJin

1999, 17(5):  7-281. 

Asbtract ( )   PDF (176KB) ( )  

Related Articles | Metrics

　AIM: To compare and analyze the homology of genes encoding histidine rich proteinII (HRPII) of different Plasmodium falciparum isolates. METHODS: Using PCR technique, the complete genes coding for HRPII of P.falciparum isolates FCC1/HN and VN isolates were amplified. PCR products were digested by HindIII/BamHI and cloned into plasmid pUC19. The recombinant plasmid HRPII/pUC19 was screened and identified by PCR and restriction analysis. The cloned HRPII genes were sequenced by Sangers method. RESULTS: HRPII genes of FCC1/HN and VN isolates were successfully amplified and cloned into pUC19. DNA sequencing showed that the coding length of HRPII gene was 1 020 bp without introns in FCC1/HN and VN isolates, however, there were ten points mutations between them. FCC1/HN isolate exhibited 98 8%, 92 2% and 98 7% homology in amino acids with isolates VN, IMTM22, and Itg2, respectively. Though the numbers of repeat sequences were different in four isolates, they had the same hydrophobic leader sequence and a single putative glycosylation site. The secondary structure analysis showed that the main antigenic determinants of four isolates were located on 5end non repeat region (amino acids 1-60). CONCLUSION: FCC1/HN isolate was highly homologous in the coding region of HRPII with VN, IMTM22, and Itg2 isolate. Four isolates exhibited similar structural characteristics and antigenic determinants in HRPII.


EFFECT OF NITROQUINE ON THE MEMBRANE PHOSPHOLIPID OF INTRAERYTHROCYTIC PLASMODIUM YOELII IN VITRO

DENGShufeng;HUYoumei

1999, 17(5):  8-284. 

Asbtract ( )   PDF (264KB) ( )  

Related Articles | Metrics

　AIM: To study the mechanism of antimalarial action of nitroquine.METHODS:Intraerythrocytic P. yoelii was cultured by the method of Trager and Jensen. The amount of [ 3H] ethanolamine incorporation was measured as an index of the phospholipid synthesis.DPH was used as a probe to measure the plasmodial fluorescent polarization.RESULTS:The incorporation of [ 3H] ethanolamine into the P.yoelii infected erthrocytes was markedly inhibited by nitroquine. The plasmodial membrane polarization and viscosity were significantly increased by nitroquine.CONCLUSION: Nitroquine could inhibit the phospholipid synthesis and decrease the membrane fluidity of P.yoelii .


SEQUENCING AND ANALYSIS OF A cDNA CLONE CO111 OF PLASMODIUM FALCIPARUM 

WANGYanni;HUANGYan;LIMing;SUNWanbang;XIEYi;LIYingjie

1999, 17(5):  9-287. 

Asbtract ( )   PDF (254KB) ( )  

Related Articles | Metrics

　AIM: To sequence and analyse a cDNA clone CO111 , reacting with immune sera obtained from rabbits immunized with Plasmodium falciparum and one McAb against P falciparum. METHODS: cDNA clone was amplified by PCR. The PCR product was purified and polished with Klenow enzyme and ligated into the M13mp18 vector (digestd by SamI) and then transformed into E coli JM109. The positive recombinant was screened out by PCR and sequenced by the PRISM Dye Primer Sequencing Kit(ABI).The sequence was analyzed by the program from Geneva University and was compared by GenBank of EMBL. RESULTS: The nucleotide sequence of this cDNA clone contains an open reading frame of 233 bp, which encodes a predicted polypeptide of 77 amino acid residues. The ratio between A+T and G+C is 3.16. The polypeptide is highly hydrophilic and flexible. Comparison among cDNA of P falciparum from GenBank of EMBL showed that no sequence identical to this cDNA was found. CONCLUSION: A novel cDNA clone reacted with the antibodies against P falciparum was isolated.


STUDIES ON IMMUNOPROTECTION IN MICE AFTER IMMUNIZATION WITH SCHISTOSOMA JAPONICUM 22.6 kDa RECOMBINANT PROTEIN *

SUChuan;MALei;WANGRongzhi;HUXuemei;CHENShuzhen;SHAOLijun;WUHaiwei;SHENLei;ZHANGZhaosong;WUGuanling

1999, 17(5):  10-291. 

Asbtract ( )   PDF (204KB) ( )  

Related Articles | Metrics

　AIM: To evaluate the immunoprotective effect of Schistosoma japonicum recombinant 22.6 kDa (rSj22.6) and Sj22.6/Sj26 GST fusion protein. METHODS:The Sj22.6/Sj26 GST fusion protein was prepared by affinity chromatography using glutathione Sepharose 4B. The purified rSj22.6 could be cleaved easily from the fusion protein with Thrombin.17 and 12 mice immunized with rSj22.6 and Sj22.6/Sj26 GST separately were each challenged with 40±1 S. japonicum cercariae.RESULTS: In BALB/c mice, the rSj22.6 and Sj22.6/Sj26 GST could induce 32.1 (P<0.005) and 34.9% (P<0.02) worm reduction, respectively, as well as 28.4% (P<0.02) and 45.1% (P<0.005) total egg reduction, respectively. CONCLUSION: Bpth rSj22.6 and Sj22.6/Sj26 GST fusion protein are partially effective against S.japonicum.


EFFECT OF LIPOSOMAL ALBENDAZOLE ON THE ULTRASTRUCTURE OF ECHINOCOCCUS GRANULOSUS CYSTS IN MICE

SHAOYingmei;LIUWenjie;WENHao

1999, 17(5):  11-293. 

Asbtract ( )   PDF (245KB) ( )  

Related Articles | Metrics

　AIM:To observe the histopathological changes of Echinococcus granulosus cysts in mice treated with liposomal albendazole and co administration with cimetidine by light microscopy and electron microscopy. METHODS:An oral dose of liposomal ABZ with different formulations was given at 200 mg/kg·d.Cimetidine was administered daily at an oral dose of 100 mg/kg·d. Sixty seven mice were orally given different drugs six days per week for a total of twelve weeks. RESULTS: The histopathological changes indicated that there were significant differences ( P <0.01) between treated groups and control group. The degeneration and necrosis of E.granulosus cysts were marked in liposomal albendazole combined with cimetidin group. CONCLUSION:Liposomal albendazole was more effective against E.granulosus cyst than albendazole. Cimetidine had an apparent synergistic effect when given in combination with liposomal albendazle.


SEQUENCE ANALYSIS OF THE MSP 1 GENE OF PLASMODIUM FALCIPARUM ISOLATES FROM HAINAN, CHINA

JIANGGangfeng;LIURuizi;ClaudiaA.Daubenberger;GerdPluschke

1999, 17(5):  12-297. 

Asbtract ( )   PDF (317KB) ( )  

Related Articles | Metrics

　AIM: To obtain the complete sequence and analyze the diversity of the MSP 1 molecule from the Chinese isolates of Plasmodium falciparum . METHODS: Genomic DNA was prepared directly from blood samples spotted on filter papers from 2 malaria patients from Baoting County, Hainan Province. PCR amplification of the target gene was carried out using 5 pairs of oligonucleotides specific for the MSP 1 gene. Direct sequencing of the target gene fragments was performed using ABI PRISM TM Dye Terminator Cycle Sequencing Ready Reaction Kit(Perkin Elmer) in a automatic ABI PRISM TM 310 Genetic Analyzer. RESULTS: For the first time, two complete sequences of the MSP1 gene from two Chinese isolates of Plasmodium falciparum were obtained. A comparison with the previously reported sequences identified them as members of the MAD20 allelic family. The deduced amino acid sequences of the MSP1 from this two Chinese isolates were identical with each other except for Blocks 2, 4 and 8. CONCLUSION: The sequences of the MSP 1 from two Chinese isolates of P. falciparum belong to the MAD20 allelic family. Minor variations through the whole sequences exist compared with the MAD20 sequence. The results provide the first evidence of the diversity of the MSP 1 molecule from Chinese isolates of Plasmodium falciparum .


DYNAMIC CHANGES IN THE EXPRESSION OF TYPE VI COLLAGEN IN MICE WITH SCHISTOSOMAL LIVER FIBROSIS 

SHIGuangfeng;XUZhaoyue;WENGXinhua;ZHUYunsong;MAJinyu

1999, 17(5):  13-301. 

Asbtract ( )   PDF (222KB) ( )  

Related Articles | Metrics

　AIM:To clarify the dynamic changes in type Ⅵ procollagen mRNA expression and collagen content in mice with schistosomal liver fibrosis.METHODS:Northern blot hybridization and immunohistochemical technique.RESULTS:The expression of α1(Ⅵ) procollagen mRNA measured by Northern blot hybridization in the liver of schistosome infected mice was elevated significantly at 8 wk post infection,peaked at 10 wk pi, slightly decreased at 12～16 wk pi,and sustained at high levels until 20 wk pi. Immunohistochemical assay revealed that the staining pattern of type Ⅵ collagen first appeared in the walls of central veins and liver sinusoids at 8 wk pi, then extended gradually to the egg granuloma and the portal tract,within and surrounding the egg granuloma to form dense reticular septum at the 12,16 and 20 wk pi, respectively.The Ⅵ collagen content as indicated by the intensity of stain peaked at 16 wk pi. CONCLUSION:Both the expression of α1(Ⅵ) procollagen and the content of collagen Ⅵ were significantly increased in the liver of mice with early schistosomal fibrosis,suggesting that the type Ⅵ collagen detection might be of importance for evaluating the intensity of schistosomal liver fibrosis.


实验研究

HUMORAL IMMUNE RESPONSE IN MICE TO HYBRID NUCLEIC ACID VACCINES CONTAINING PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1 BLOCK 17 BASED GENE *

MIAOJun;LIXun;XUECaifang;ZHENRongfen;LIUZhongxiang;QINEnqiang;YUQigui

1999, 17(5):  14-304. 

Asbtract ( )   PDF (251KB) ( )  

Related Articles | Metrics

　AIM: To analyse the humoral immune response in mice to nucleic acid vaccines ( VR1012/HG MSP1 17 for intracellular expression or VR1012/TPA/HG MSP1 17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL 1 and TT. METHODS: BALB/c or C 57 BL/6 mice received three times intramuscular immunization with 200 μg/100 μl or 100 μg/100 μl of VR1012/HG MSP1 17 or VR1012/TPA/HG MSP1 17 per mouse each time. Anti HG or anti MSP1 17 antibodies were monitored by indirect ELISA. RESULTS: BALB/c and C 57 BL/6 mice immunized with 100 μg/100 μl of VR1012/HG MSP1 17 per mouse raised significantly anti HG and anti MSP1 17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200 μg/100 μl of VR1012/HG MSP1 17 per mouse raised higher anti HG antibodies but not anti MSP1 17 antibodies. BALB/c mice immunized with 200 μg/100 μl of VR1012/TPA/HG MSP1 17 per mouse raised low level of anti HG antibodies only. CONCLUSION: VR1012/HG MSP1 17 is more immunogenic than VR1012/TPA/HG MSP1 17.



STUDIES ON ALLELIC ZYMOGRAM OF PERIODIC BRUGIA MALAYI FROM ZHEJIANG PROVINCE 

DENGShanshan;WANGPengpeng;ZENGXiaopeng;YANXiaolan;Director:CHENCuie

1999, 17(5):  15-307. 

Asbtract ( )   PDF (94KB) ( )  

Related Articles | Metrics

　AIM:To study the allelic zymogram of periodic B.malayi from Zhejiang Province. METHODS:180 adult worms(60,♀120),32 000 microfilaria and 1 500 infective larvae of periodic B.malayi were examined for the isoenzymes of 14 enzymes by horizontal starch gel electrophoresis with 14 different isomyme.RESULTS:Twenty seven allelic loci were found in three developing stages of B. malayi , most of them were homozygotes,however, two of them(MPI,MDH)were heterozygotes(7.4%).17,19 and 9 loci were presented in adult worms,microfilariae and infective larvae, respectively.At the same time, the allelic zymogram of B.malayi from Guizhou Province was also examined.CONCLUSION:The allelic zymogram of periodic B. malayi from Zhejiang Province was similar to that of periodic B. malayi from Guizhou Province but passaged in the laboratory.


动物模型

ESTABLISHMENT OF A RABBIT MODEL OF ACANTHAMOEBA KERATITIS

DENGXinguo;GUOXue;PANGGuangren;TIANXiaoli

1999, 17(5):  16-310. 

Asbtract ( )   PDF (154KB) ( )  

Related Articles | Metrics

　AIM: To establish an animal model of Acanthamoeba keratitis. METHODS:Six New Zealand white rabbits were each injected intrastromally with Acanthamoeba suspension 3 days after subconjunctival injection with dexamethasone. RESULTS:All of the 6 rabbits developed keratitis. Acanthamoeba protozoa were identified by the methods of corneal scraping with 10% potassium hydroxide wet mount examined under microscope,corneal protozoa culture and pathological section examination. CONCLUSION:A rabbit model of Acanthamoeba keratitis was established.