›› 1999, Vol. 17 ›› Issue (5): 15-307.

• 实验研究 • Previous Articles     Next Articles

STUDIES ON ALLELIC ZYMOGRAM OF PERIODIC BRUGIA MALAYI FROM ZHEJIANG PROVINCE 

DENG Shanshan;WANG Pengpeng;ZENG Xiaopeng;YAN Xiaolan;Director:CHEN Cuie   

  1. Institute of Parasitic Diseases;Zhejiang Academy of Medical Sciences;Hangzhou 310013
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-10-30 Published:1999-10-30

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Abstract: 　AIM:To study the allelic zymogram of periodic B.malayi from Zhejiang Province. METHODS:180 adult worms(60,♀120),32 000 microfilaria and 1 500 infective larvae of periodic B.malayi were examined for the isoenzymes of 14 enzymes by horizontal starch gel electrophoresis with 14 different isomyme.RESULTS:Twenty seven allelic loci were found in three developing stages of B. malayi , most of them were homozygotes,however, two of them(MPI,MDH)were heterozygotes(7.4%).17,19 and 9 loci were presented in adult worms,microfilariae and infective larvae, respectively.At the same time, the allelic zymogram of B.malayi from Guizhou Province was also examined.CONCLUSION:The allelic zymogram of periodic B. malayi from Zhejiang Province was similar to that of periodic B. malayi from Guizhou Province but passaged in the laboratory.

Key words: Periodic Brugia malayi, allelic zymogram, horizontal starch gel elctrophoresis