›› 2003, Vol. 21 ›› Issue (6): 10-356.

• 论著 • Previous Articles     Next Articles

Study on the Subcloning, Expression and Immunoprotection with Schistosoma japonicum CAI Gene

LUO Xiu-ju1,YUAN Shi-shan1,YI Xin-yuan1 *,ZENG Xian-fang1,ZHANG Shun-ke1,TANG Lian-fei1,CAI Chun1,ZHANG Jie1,Larry McReynolds2
  

  1. 1 Department of Parasitology;,Xiangya School of Medicine,Central South University,Changsha 410078 2 New England Biolabs,Inc. MA 01905 USA
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-12-30 Published:2003-12-30

PDF (PC)

Abstract: 　Objective To subclone and express the new gene of Schistosoma japonicum (Sj) CAI and evaluate the immunoprotective effect of the recombinant molecule. Methods The cDNA of SjCAI gene was subcloned into expression vector pGEX-5X-3 to form recombinants which were then used to transform to E.coli strain ER 2566. Expression was induced by IPTG. The mice were vaccinated with the expressed protein and the immunoprotective effect was tested. Results Fusion protein of SjGST-CAI was highly expressed in E. coli as inclusion bodies. The worm reduction rate and the liver egg reduction rate in vaccination group of SjGST-CAI were 29.87% and 63.71%, respectively. Conclution SjCAI gene can be highly expressed in E .coli after subcloning into pGEX-5X-3 vector and the expressed fusion protein can induce immunoprotective effect against Sj in mice.

Key words: Schistosoma japonicum, CAI protein, expression, immunoprotection