Detection and analysis of fecal egg and serum IgG antibody in 146 suspected cases of Clonorchis sinensis infection in Guangxi

doi:10.12140/j.issn.1000-7423.2019.06.022

Abstract

Abstract:

In order to determine the endemic status and develop a diagnostic method for Clonorchis sinensis infection, the fecal eggs of C. sinensis and Haplorchis taichui and the specific IgG antibody in the sera of patients with suspected C. sinensis infections in Guangxi was examined. The fecal samples of suspected C. sinensis infected cases were collected from the Department of Preventive Health Examination, Guangxi Center for Disease Prevention and Control from March to September, 2017. Kato-Katz thick smears were prepared from each stool sample in triplicate. The eggs of C. sinensis or H. taichui were examined and counted (EPG, egg per gram stool). Blood samples from fecal positive patients were collected and the serum IgG antibodies against C. sinensis were detected by indirect ELISA. The correlation between EPG and A₄₅₀ value of IgG antibody was analyzed. The maximum EPG is 21 502 and the minimum EPG is 8 among 146 eggs-positive fecal samples. The total positive rate of C. sinensis IgG antibody was 84.9% (124/146). The antibody positive rates in male and female patients were 86.0% (111/129) and 76.5% (13/17), respectively, without significant difference (P > 0.05). There was a positive correlation between fecal egg EPG and serum C. sinensis IgG A₄₅₀ value (r_s = 0.436, P < 0.01). The positive rates of IgG antibody in patients with mild, moderate, and severe infection were 78.4% (69/88), 96.0% (48/50) and 87.5% (7/8), respectively, and showed significant difference among them (P < 0.05). The geometric mean of fecal EPG in individuals with C. sinensis IgG positive and negative were 736 and 204, respectively, with significant difference (P < 0.01). There is a positive correlation between fecal egg count and C. sinensis IgG antibody titer in Guangxi, where C. sinensis and H. taichui are co-infected. However, there was false negative in the detection of C. sinensis infection by fecal examination alone, serological antibody detection could increase the diagnostic of C. sinensis infection.

Key words: Clonorchis sinensis, Haplorchis taichui, Eggs, IgG antibody, Co-infection

CLC Number:

R383.22

Xue-mei ZENG, Zhi-hua JIANG, Ji-qing SHEN, Qing-li YANG. Detection and analysis of fecal egg and serum IgG antibody in 146 suspected cases of Clonorchis sinensis infection in Guangxi[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(6): 730-732.

Figures/Tables 1

References 15

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