Analysis of Blastocystis hominis infection in long-term hospitalized patients in Tianmen City

doi:10.12140/j.issn.1000-7423.2023.01.020

Abstract

Abstract:

Basic information (gender, age, fecal characteristics, occult-blood test results, clinical signs and symptoms, etc) of long-term hospitalized patients in Tianmen First People’s Hospital during June—September 2021 collected. The fecal samples of patients were collected and examined under microscope by direct smear with normal saline. The DNA were extracted from the suspected Blastocystis hominis positive fecal samples. Specific primers of B. hominis BhRDr and RD5 were used to amplify the target bands, and the positive fecal samples were sent to Norsay Genomics Research Center for sequencing. Giardia lamblia and fragile bicarbonate amoeba infection were also observed in the fecal samples of the patients. Anticoagulant whole blood and non-anticoagulant venous blood were collected for blood analysis. Among the 857 patients, 42 cases were positive for B. hominis, with a positive rate of 4.90%. The positive rate of males was 4.27% (19/445) and that among the females was 5.58% (23/412). There was no significant difference between the gender groups (χ² = 0.791, P < 0.05). In different age groups, the positive rate was the highest in the ≥ 61 years old group (6.38%, 22/345). There was no significant difference in positive rates among different age groups (χ² = 2.700, P > 0.05). Among different fecal characters, the positive rate of the loose stool samples was the highest (18.37%, 27/147) (χ² = 98.930, P < 0.05). The positive rate was 6.72% (9/134) in patients with hematochezia and 4.56% (33/723) in patients without hematochezia, with no significant difference (χ² = 1.123, P > 0.05). Among different clinical symptoms, patients with abdominal pain and diarrhea had the highest positive rate (4/11) (χ² = 53.956, P < 0.05). Six patients were infected with B. hominis mixed with G. Lamblia, and two were infected with amoeba fragile. The red blood cell count and hemoglobin of B. hominis infected patients were (3.97 ± 7.39) × 10¹²/L and (119.02 ± 21.49) g/L, both of which were lower than that of the non-infected patients [(4.27 ± 0.44) × 10¹²/L, (126.52 ± 14.30) g/L] (t = 2.556, 2.235; P < 0.05). There were four genotypes of B. hominis (ST1, ST3, ST6 and ST7), and ST3 was the dominant one, accounting for 57.1% (24/42).

Key words: Blastocystis hominis, Fecal traits, Occult blood, Co-infection

CLC Number:

R382

ZHU Mingchao, ZHU Ya, ZHAO Jianzhong, YUAN Huizhen. Analysis of Blastocystis hominis infection in long-term hospitalized patients in Tianmen City[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 121-124.

Figures/Tables 2

References 21

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	病例数	感染者人数	感染率/%	χ²值	P值
年龄组/岁
0~18	50	2	4.00	2.700	> 0.05
19~60	462	18	3.90
≥ 61	345	22	6.38
性别
男	445	19	4.27	0.791	> 0.05
女	412	23	5.58
粪样性状
成形便	356	2	0.56	98.930	< 0.01
半成形便	185	1	0.54
软便	151	9	5.96
稀便	147	27	18.37
水样便	18	3	3/18
隐血试验
未便血	723	33	4.56	1.123	> 0.05
便血	134	9	6.72
临床症状
食欲不振	4	1	1/4	53.956	< 0.01
腹泻	24	8	33.33
腹胀	7	2	2/7
腹痛或腹部不适	21	1	4.76
腹痛且腹泻	11	4	4/11
腹胀、腹痛且腹泻	3	0	0
无明显症状	787	26	3.30
检出其他寄生虫
检出蓝氏贾第鞭毛虫	8	6	6/8	57.484	< 0.01
检出脆弱双核阿米巴	3	2	2/3	0.155	> 0.05

实验室检查项目	参考范围	感染者数值	非感染者数值	t值	P值	95％CI 上限	95％CI 下限
白细胞计数	（3.5~9.5） × 10⁹/L	（6.40 ± 3.07） × 10⁹/L	（6.31 ± 2.09） × 10⁹/L	-0.251	0.802	-1.051	0.881
红细胞计数	（ 3.5~5.5） × 10¹²/L	（3.97 ± 7.39） × 10¹²/L	（4.27 ± 0.44） × 10¹²/L	2.556	0.014	0.062	0.526
血红蛋白	（120~160） g/L	（119.02 ± 21.49） g/L	（126.52 ± 14.30） g/L	2.235	0.031	0.731	14.257
血小板计数	（125~350） × 10⁹/L	（192.43 ± 86.65） × 10⁹/L	（216.20 ± 63.01） × 10⁹/L	1.754	0.086	-3.552	51.097
丙氨酸氨基转移酶	（11~16） IU/L	（23.32 ± 25.72） IU/L	（23.90 ± 13.01） IU/L	0.145	0.885	-7.583	8.757
天冬氨酸氨基转移酶	（35~56） IU/L	（28.01 ± 23.03） IU/L	（27.20 ± 15.98） IU/L	-0.195	0.846	-9.156	7.540
C反应蛋白	≤ 6 mg/L	（15.16 ± 30.13） mg/L	（6.41 ± 21.38） mg/L	-1.77	0.085	-18.760	1.253