Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City

doi:10.12140/j.issn.1000-7423.2021.01.008

CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2021, Vol. 39 ›› Issue (1): 55-60.doi: 10.12140/j.issn.1000-7423.2021.01.008

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Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City

QIAN Ke¹(), HU Zhu-hua¹, WU Xiu-ping², PENG Guo-hua¹, YU Ying-fang², CHU Yan-hong², ZHOU Xian-feng¹, XU Dan¹, TENG Xue-jiao², LI Hui³, TIAN Li-guang²^,^*()

1 The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang 330038, China
2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
3 Nanchang Municipal Health Commission, Nanchang 330038, China

Received:2020-09-28 Revised:2020-11-25 Online:2021-02-28 Published:2021-03-10
Contact: TIAN Li-guang E-mail:qkqk2003@sina.com;jztlg@126.com
Supported by:
National Natural Science Foundation of China(81473022);Scientific Research Project of Shanghai Municipal Health and Family Planning Commission(291440499);National Key Research and Development Program(2016YFC1202700);National Key Research and Development Program(2016YFC1202701);Science and Technology Program of Jiangxi Provincial Health and Family Planning Commission(20186023);Key R&D Project of Jiangxi Provincial Department of Science and Technology(20192BBGL70008)

Abstract

Abstract:

Objective To determine the prevalence and genotype of Blastocystis hominis among HIV-positive individuals in Nanchang City. Methods A cross-sectional questionnaire survey was made among registered HIV-positive individuals in Nanchang City during May and September in 2016. Blood and fecal samples were collected. The level of CD4 ⁺ T cells in blood was detected. DNA genome was extracted from the fecal samples to amplify the small subunit ribosomal ribonucleic acid (SSU rDNA) of B. hominis. The positive products were sequenced and aligned to confirm the diagnosis, and phylogenic tree was constructed for genotyping. Results A total of 505 subjects were surveyed, and 505 fecal samples and 475 blood samples were collected. The infection rate of B. hominis was 4.16% (21/505) in the HIV/AIDS individuals, 4.22% (18/427) in the males and 3.85% (3/78) in the females, with no significant difference between males and females (P > 0.05). The infection rates in the HIV/AIDS populations from Methadone outpatient clinics of the Center for Disease Control, municipal infectious disease hospitals, and prison hospitals were 3.74% (4/107), 1.62% (5/308) and 13.33% (12/90), respectively. The infection rate in those from prison hospitals was significantly higher than the other two (P < 0.05). The infection rates in the HIV/AIDS populations with CD4 ⁺ T cell count > 400/μl and ≤ 400/μl were 3.29% (5/152) and 4.64% (15/323), respectively (P > 0.05). Sequencing results revealed prevalence of subtypes ST1 (4.76%, 1/21), ST3 (90.48%, 19/21), and ST7 (4.76%, 1/21) in Nanchang City. Conclusion There is high prevalence of B. hominis in HIV/AIDS cases from prison hospitals in Nanchang, and ST3 is the predominant subtype.

Key words: Blastocystis hominis, HIV/AIDS, Genotype characterization, Nanchang

CLC Number:

R382.9

QIAN Ke, HU Zhu-hua, WU Xiu-ping, PENG Guo-hua, YU Ying-fang, CHU Yan-hong, ZHOU Xian-feng, XU Dan, TENG Xue-jiao, LI Hui, TIAN Li-guang. Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(1): 55-60.

Figures/Tables 2

References 26

[1]	Stenzel DJ, Boreham PF. Blastocystis hominis revisited[J]. Clin Microbiol Rev, 1996,9(4):563-584. doi: 10.1128/CMR.9.4.563-584.1996 pmid: 8894352
[2]	Tan KSW. Blastocystis in humans and animals: new insights using modern methodologies[J]. Vet Parasitol, 2004,126(1/2):121-144. doi: 10.1016/j.vetpar.2004.09.017
[3]	Eroglu F, Koltas IS. Evaluation of the transmission mode of B. hominis by using PCR method[J]. Parasitol Res, 2010,107(4):841-845. doi: 10.1007/s00436-010-1937-4
[4]	Meloni D, Sanciu G, Poirier P, et al. Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy[J]. Parasitol Res, 2011,109(3):613-619. doi: 10.1007/s00436-011-2294-7
[5]	Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp.[J]. Clin Microbiol Rev, 2008,21(4):639-665. doi: 10.1128/CMR.00022-08 pmid: 18854485
[6]	Hu Y, Chen LY, Li YW. Intestinal protozoan infections among 451 patients with malignant cancer[J]. Mod Prev Med, 2015,42(20):3816-3817, 3833. (in Chinese)
	( 胡缨, 陈丽阳, 李艳文. 451例恶性肿瘤患者中肠道原虫感染情况研究[J]. 现代预防医学, 2015,42(20):3816-3817, 3833.)
[7]	Kurniawan A, Karyadi T, Dwintasari SW, et al. Intestinal parasitic infections in HIV/AIDS patients presenting with diarrhoea in Jakarta, Indonesia[J]. Trans R Soc Trop Med Hyg, 2009,103(9):892-898. doi: 10.1016/j.trstmh.2009.02.017 pmid: 19327806
[8]	Roberts T, Stark D, Harkness J, et al. Update on the pathogenic potential and treatment options for Blastocystis sp.[J]. Gut Pathog, 2014,6:17. doi: 10.1186/1757-4749-6-17 pmid: 24883113
[9]	Tian LG, Cheng GJ, Chen JX, et al. Survey on co-infection with HIV and intestinal parasites in high prevalence areas of HIV/AIDS, China[J]. Chin J Schisto Control, 2012,24(2):168-172. (in Chinese)
	( 田利光, 程国金, 陈家旭, 等. 我国农村HIV/AIDS高流行地区肠道寄生虫感染现况调查[J]. 中国血吸虫病防治杂志, 2012,24(2):168-172.)
[10]	Tian LG, Chen JX, Cheng GJ, et al. Survey on Blastocystis hominis infection in HIV positive individuals in Fuyang City, Anhui Province[J]. Chin J Schisto Control, 2012,24(3):303-306, 310. (in Chinese)
	( 田利光, 陈家旭, 程国金, 等. 安徽阜阳HIV阳性者合并人芽囊原虫感染及其影响因素分析[J]. 中国血吸虫病防治杂志, 2012,24(3):303-306, 310.)
[11]	Khoshnood S, Rafiei A, Saki J, et al. Prevalence and genotype characterization of Blastocystis hominis among the baghmalek people in southwestern Iran in 2013—2014[J]. Jundishapur J Microbiol, 2015,8(10):e23930. doi: 10.5812/jjm.23930 pmid: 26587213
[12]	Zali MR, Mehr AJ, Rezaian M, et al. Prevalence of intestinal parasitic pathogens among HIV-positive individuals in Iran[J]. Jpn J Infect Dis, 2004,57(6):268-270. pmid: 15623953
[13]	Roberts T, Stark D, Harkness J, et al. Update on the pathogenic potential and treatment options for Blastocystis sp.[J]. Gut Pathog, 2014,6:17. doi: 10.1186/1757-4749-6-17 pmid: 24883113
[14]	Xie RH, Chen GX, Ouyang SS. Analysis status of intestinal parasite infection among HIV/AIDS patients in Hengyang[J]. Chin J Immunol, 2015,31(5):695-697. (in Chinese)
	( 谢荣华, 陈高翔, 欧阳珊珊. 衡阳地区HIV/AIDS患者合并肠道寄生虫感染现状分析[J]. 中国免疫学杂志, 2015,31(5):695-697.)
[15]	Tian LG, Wang TP, Lv S, et al. HIV and intestinal parasite co-infections among a Chinese population: an immunological profile[J]. Infect Dis Poverty, 2013,2:18. doi: 10.1186/2049-9957-2-18 pmid: 23971713
[16]	Stensvold R, Brillowska-Dabrowska A, Nielsen HV, et al. Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction[J]. J Parasitol, 2006,92(5):1081-1087. doi: 10.1645/GE-840R.1 pmid: 17152954
[17]	Alfellani MA, Taner-Mulla D, Jacob AS, et al. Genetic diversity of Blastocystis in livestock and zoo animals[J]. Protist, 2013,164(4):497-509. doi: 10.1016/j.protis.2013.05.003
[18]	Kesuma Y, Firmansyah A, Bardosono S, et al. Blastocystis ST-1 is associated with irritable bowel syndrome-diarrhoea (IBS-D) in Indonesian adolescences[J]. Parasite Epidemiol Control, 2019,6:e00112. doi: 10.1016/j.parepi.2019.e00112 pmid: 31528737
[19]	Singh A, Priyadarshi K, Rai T, et al. A case report of Blastocystis infection and Steven Johnson’s syndrome[J]. Trop Biomed, 2019,36(4):987-992. pmid: 33597468
[20]	Zhou YL, Wu L, Yin Q, et al. Infections of Clostridium difficile, Blastocystis hominis and Cryptosporidium spp. in patients with chronic diarrhea[J]. Chin J Parasitol Parasit Dis, 2019,37(6):723-726. (in Chinese)
	( 周亚玲, 吴亮, 阴晴, 等. 慢性腹泻患者艰难梭菌、人芽囊原虫和隐孢子虫感染的研究[J]. 中国寄生虫学与寄生虫病杂志, 2019,37(6):723-726.)
[21]	de Melo GB, Malta FDM, Maruta CW, et al. Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil[J]. Parasite Epidemiol Control, 2019,7:e00124. doi: 10.1016/j.parepi.2019.e00124 pmid: 31872093
[22]	Kök M, Çekin Y, Çekin AH, et al. The role of Blastocystis hominis in the activation of ulcerative colitis[J]. Turk J Gastroenterol, 2019,30(1):40-46. doi: 10.5152/tjg.2018.18498 pmid: 30644363
[23]	Stensvold CR. Blastocystis: genetic diversity and molecular methods for diagnosis and epidemiology[J]. Trop Parasitol, 2013,3(1):26-34. doi: 10.4103/2229-5070.113896 pmid: 23961438
[24]	Teng XJ, Chu YH, Zhai CC, et al. The epidemiological characteristics and influencing factors for Blastocystis hominis infection among human immunodeficiency virus seropositive individuals in Tengchong of Yunnan Province[J]. Chin J Parasitol Parasit Dis, 2018,36(2):129-134. (in Chinese)
	( 滕雪娇, 储言红, 翟铖铖, 等. 云南腾冲人类免疫缺陷病毒携带者人芽囊原虫感染的流行病学特征及影响因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(2):129-134.)
[25]	Alfellani MA, Stensvold CR, Vidal-Lapiedra A, et al. Variable geographic distribution of Blastocystis subtypes and its potential implications[J]. Acta Trop, 2013,126(1):11-18. doi: 10.1016/j.actatropica.2012.12.011 pmid: 23290980
[26]	Stensvold CR, Clark CG. Current status of Blastocystis: a personal view[J]. Parasitol Int, 2016,65(6 pt b):763-771. doi: 10.1016/j.parint.2016.05.015 pmid: 27247124

Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City

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