Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi

doi:10.12140/j.issn.1000-7423.2019.03.014

Abstract

Abstract:

Objective To investigate the co-infection rate and the genotype of Giardia lamblia among HIV/AIDS patients in Guangxi Autonomous Region. Methods Fecal specimens were collected from 285 HIV/AIDS patients and 303 HIV-negative people from Guangxi Autonomous Region. DNA were extracted from the feces and triosephosphate isomerase (TPI) gene of G. lamblia was amplified by nested PCR to determine the infection of G. lamblia. The amplified TPI PCR products were sequenced and the obtained sequences were aligned and analyzed by BLAST search. Results The overall co-infection rate of G. lamblia with HIV/AIDS was 2.8% (8/285). There was no G. lamblia infection detected in people without HIV-AIDS (P < 0.01). However, no significant risk factors were found to be related to the infection of G. lamblia among HIV-AIDS patients such as age, gender, sanitation, antiretroviral treatment or clinical manifestations (diarrhea, CD4⁺T lymphocyte level). Seven samples were identified to be zoonotic assemblage B and 6 had 100% sequence homology with the reported human-derived isolate. The other isolate was assemblage C and sequence identical to the reported human-derived isolate in our previous study. Conclusion HIV-AIDS patients have higher co-infection rate with G. lamblia than people without HIV infection. The major genotype of infected G. lamblia was assemblage B.

Key words: Giardia lamblia, Assemblage, Nested PCR, HIV/AIDS

CLC Number:

R531.73

Hua LIU, Ning XU, Yu-juan SHEN, Yuan HU, Jian-ping CAO. Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(3): 321-325.

Figures/Tables 3

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风险因素 Risk factors	人数 No. of people	感染人数 G. lamblia infected	阳性率/% Positive rate/%	χ²值 χ² value	P值 P value
性别Gender
男Male	216	7	3.2	0.134	> 0.05
女Female	69	1	1.4
年龄组Age group
<40	93	1	1.1		> 0.05
40~60	113	4	3.5
>60	79	3	3.8
职业Occupation
农民 Farmer	217	7	3.2	0.118	> 0.05
其他 Others	68	1	1.5
CD4⁺ T细胞数
CD4⁺ lymphocyte count
CD4⁺ ≥ 200	49	1	2.0	0.052	> 0.05
CD4⁺ < 200	119	5	4.2
是否进行抗病毒治疗Antiretroviral treatment
是Yes	119	4	3.4	0	> 0.05
否No	131	4	3.1
感染途径Transmission route
性传播 Sexual	240	5	2.1	1.48	> 0.05
其他Others	45	3	6.7
饮用生水Drinking unboiled water
是Yes	19	0	0		> 0.05
否No	266	8	3.0
是否发生腹泻Diarrhea
是Yes	33	0	0		> 0.05
否No	252	8	3.2