›› 2014, Vol. 32 ›› Issue (1): 19-76-77.

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Identification of Mammalian Blood Meals in Anopheline Mosquitoes from Four Counties of Yunnan Province by Multiple PCR

ZHANG Jing-wei1，JIANG Jin-yong2，WANG Xue-zhong2，ZENG Xu-can2，ZHENG Yu-ting2，ZHOU Hong-ning1，2 *，LI Hong-bin3，LONG Zhi4，YANG Tai-zhuan5   

  1. 1 Puer Branch of the Institute of Pathogens and Vectors，Dali University, Puer 665000, China; 2 Yunnan Institute of Parasitic Diseases, Puer 665000, China; 3 Centre for Disease Control and Prevention of Xishuangbanna Prefecture, Jinghong 666100, China; 4 Centre for Disease Control and Prevention of Zhaotong Prefecture, Zhaotong 657000, China; 5 Centre for Disease Control and Prevention of Yuanjiang County, Yuanjiang 653300, China
  • Online:2014-02-28 Published:2014-05-12

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Abstract:  【Abstract】 From June to August 2012, the blood-sucking mosquitoes were captured around cattle-sheds and human houses in Yuanjiang County, Qiaojia County, Yongshan County, and Jinghong City of Yunan Province. Blood samples from mosquitoes were collected on filter paper. Multiplex PCR assay was used to detect the blood meal samples. Among the 145 mosquitoes captured, 123 were Anopheles sinensis（84.8%） and 22 A. minimus（15.2%）. Among the blood samples, corresponding bands were amplified in 134 samples. The result showed that the blood meals were from pigs （n=104）, cows （n=22）, dogs （n=4）, human （n=2）, cow and pig （n=1）, pig and human （n=1）. Human blood index of A. sinensis and A. minimus was 0.018 and 0.045, respectively.

Key words: Anopheles, Blood meal, Multiplex PCR, Human blood index, Yunnan