Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border

doi:10.12140/j.issn.1000-7423.2024.01.019

Abstract

Abstract:

To understand the host-feeding habits of Anopheles minimus, the main malaria vector at China-Myanmar border areas, mosquitoes were captured from May to July 2021 in Nabang Town, Yingjiang County, Yunnan Province by the ultraviolet mosquito trap method. The captured mosquitoes were identified by morphology. The blood-sucking status of female mosquitoes was recorded: the female mosquitos with scarlet abdomens were regarded as blood-filled mosquitos. The genomic DNA of the mosquitoes identified as An. minimus were extracted, and the cytochrome C oxidase subunit 1 gene (cox1) were amplified by PCR. The sequence comparison of the positive products was performed to determine the mosquito species. Subspecies identification was based on multiplex PCR amplification of the second internal transcribed spacer (ITS2) sequence of ribosomal DNA. The blood sources identification of blood-filled female mosquitos was based on multiplex PCR amplification of cytochrome b (cytb) of mitochondrial DNA. A total of 3 419 mosquitoes were captured and 303 An. minimus were identified by morphology initially. A total of 245 mosquitoes were cox1 positive by PCR, and the sequences were over 99% identical to An. minimus (GenBank accession number: GQ259177.1). ITS2 multiplex PCR amplification results showed that all 245 An. minimus mosquitos were type A. Cytb multiplex PCR amplification results of 20 blood-filled female mosquitos showed that the blood sources of 4 mosquitos were from humans, 14 were from pigs and 2 were from cows. The human, pig, and cow blood index were 0.2 (4/20), 0.7 (14/20) and 0.1 (2/20), respectively. This study showed that the dominant Anopheles population in Yingjiang County, Yunnan Province were An. minimus, whose subspecies types were all type A. Their preferred host includes both animals and humans. The risk of malaria transmission was high.

Key words: Anopheles minimus, Host-feeding habits, China-Myanmar border

CLC Number:

384.111

ZOU Wenjian, WU Linbo, XU Xiang, TANG Yerong, SUN Xiaodong, ZENG Xucan. Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 125-128.

Figures/Tables 4

References 20

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引物名称	序列（5′→3′）	产物长度/bp
蚊种鉴定
cox1-F	GGAGGATTTGGAAATTGATTGATTAGTTC	500
cox1-R	CCCGGTAAAATTAAAATATAAACTTC
微小按蚊亚种鉴定
ITS2-F	ATCACTCGGCTCATGGATCG
ITS2-A-R	GGGCGCCATGTAGTTAGAGTTG	184
ITS2-C-R	GGTTGCCCACTCAATACGGGTG	509
蚊胃血源鉴定
cytb-hum-F	GGCTTACTTCTCTTCATTCTCTCCT	334
cytb-pig-F	CCTCGCAGCCGTACATCTC	453
cytb-cow-F	CATCGGCACAAATTTAGTCG	561
cytb-dog-F	GGAATTGTACTATTATTCGCAACCAT	680
cytb-R	GGTTGTCCTCCAATTCATGTTA