CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2020, Vol. 38 ›› Issue (3): 286-292.doi: 10.12140/j.issn.1000-7423.2020.03.005
• ORIGINAL ARTICLES • Previous Articles Next Articles
DENG Wang-ping1, HONG Qing-hua2, XU Bin1, WANG Sheng-lin1, WANG Li-ping1, XU Jing1, HU Wei1,2, ZHOU Xiao-nong1,*()
Received:
2020-03-13
Online:
2020-06-30
Published:
2020-07-07
Contact:
Xiao-nong ZHOU
E-mail:zhouxn1@chinacdc.cn
Supported by:
CLC Number:
DENG Wang-ping, HONG Qing-hua, XU Bin, WANG Sheng-lin, WANG Li-ping, XU Jing, HU Wei, ZHOU Xiao-nong. Development and preliminary evaluation of a rapid visualization detection method for circulating nucleic acids of Schistosoma japonicum based on RPA-LFD[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(3): 286-292.
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URL: https://www.jsczz.cn/EN/10.12140/j.issn.1000-7423.2020.03.005
Fig. 1
Optimization of the reaction conditions of RPA-LFD assay for S. japonicum genomic DNA A: The detection results at different reaction temperatures; B: The detection results after different reaction times. 1-7: Reaction temperature of 20, 25, 30, 35, 40, 45 and 50 ℃, respectively; 6-11: Reaction time of 0, 5, 10, 15, 20, 25 and 30 min, respectively
Fig. 2
Evaluation of the sensitivity and specificity of RPA-LFD assay for detecting S. japonicum genomic DNA A:Results of RPA-LFD assay for detecting gradient concentrations of S. japonicum genomic DNA; B: Results of RPA-LFD assay for detecting genomic DNA from different trematodes. 1-7: The amount of S. japonicum genomic DNA was 10-1, 10-2, 10-3, 10-4, 10-5, 10-6 and 10-7 ng, respectively; 8, 9: ddH2O; 10-14: Genomic DNA (1 ng) from C. sinensis, P. westermani, S. haematobium, S. mansoni, and S. japonicum, respectively
Fig. 3
The performance of RPA-LFD in detecting S. japonicum circulating DNA fragment in serum of infection mouse A: The results of RPA-LFD for detecting circulating DNA in mice dummy positive sera; B: The results of RPA-LFD for detecting circulating DNA in mouse serum with S. japonicum infection; 1: ddH2O; 2-6: The amount of S. japonicum genomic DNA in mice dummy positive sera was 100, 10, 1, 0.1, 0.01 ng, respectively; 7-10: DNA from mouse serum before infection and on days 7, 21 and 35 d after infection, respectively
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