›› 2012, Vol. 30 ›› Issue (5): 20-415-417.

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Preparation of Agar-Paraffin Double-Embedded Longitudinal Sections of Schistosoma japonicum

WANG Ke-geng1,ZENG Qing-ren2,ZHANG Yu-kuai1,ZHOU Jun3,CAI Li-ting,LIANG Yu1,LIU Yan1,2 *   

  1. 1 Department of Parasitology,School of Medicine,University of South China,Hengyang 421001,China;2 Cell and Molecular Biological Experiment Center,Xiangya School of Medicine,Central South University,Changsha 410013,China;3 Experiment Center of the Third Affiliated Hospital,Central South University,Changsha 410013,China
  • Online:2012-10-30 Published:2013-02-05

Abstract: 【Abstract】  Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block, then dehydrated with alcohol, isobutyl alcohol and n-butyl alcohol. Various staining procedures can be conducted after conventional sectioning and dewaxing. Complete longitudinal serial sections of the pre-embedded worms can be obtained, and the desired sections can be easily located accurately.

Key words: Schistosoma japonicum, Agar-paraffin double-embedded, Longitudinal section