›› 2010, Vol. 28 ›› Issue (3): 3-175.

• 论著 • Previous Articles     Next Articles

Screening and Characterization of Peptides Specifically Binding to the Schistosomulum Tegument of Schistosoma japonicum

WEI Qi1，2， LIU Yan2， ZENG Qing-ren2， CHEN Yu-xiao1*， ZHOU Jun3， LIU Bi-yuan1，2，ZOU Yan4， YANG Sheng-hui2， CAI Li-ting2， LI Li-xin1， LAN Ling-mei2   

  1. 1 Department of Immunology，Xiangya Medical School，the Central South University，Changsha 410013，China；2 Cell ＆ Molecular Biological Experiment Xiang Ya School of Medicine Center，the Central South University，Changsha 410013，China；3 the Third Xiangya Hospital of Central South University，Changsha 410013，China；4 Hunan Traditional Chinese Medical College，Zhuzhou 412012，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-30 Published:2010-06-30

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Abstract:

【Abstract】 Objective To screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum tegument of Schistosoma japonicum. Methods A 12 phage-display peptide library was screened with the S. japonicum schistosomula as the target cells for biopanning by degrees, positive clones picked randomly were deduced by DNA sequencing. According the sequencing result, immunohistochemical staining was performed to determine the specificity of the phages to the tegument. To test their targeting efficacy, the interested phage clones were infused back to the mice infected with S. japonicum, mice were sacrificed 2.5 hours later, and the phage distribution in the liver and the tegument of schistosomula was appraised, respectively. Results After 3 rounds of biopanning, the phage recovery rate increased from 0.77×10-8 to 0.75×10-5, indicating that the phage library was successfully enriched in the tegument of schistosomula. Seventy-five percent （15/20） of the analyzed sequences were identical with a sequence of QHPRIRKOOOOO. The immunohistochemical stainings showed this sequence specifically binding to the tegument. In vivo titering displayed that this sequence selectively targeted the tegument. Conclusion The peptide of QHPRIRKOOOOO specifically binds to the schistosomulum tegument.

Key words: Schistosoma japonicum, Schistosomulum, Phage display, Tegument, Target